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oai:www.ideals.illinois.edu:2142/24310 2023-07-10 col_2142_14797 col_2142_5131 com_2142_14796 com_2142_397 com_2142_5130

Loor, Juan J. Li, Jing 2011-05-25T14:50:57Z 2011-05-25T14:50:57Z 2011-05-25T14:50:57Z 2011-05 Trans-10, cis-12-conjugated linoleic acid (trans-10, cis-12-CLA) has been reported to reduce body fat mass but accompanied by increased liver mass. However, the cellular and molecular mechanisms have been unknown. Our study investigated the effects of trans-10, cis-12-CLA supplementation on hepatic gene expression in growing male mice by measuring mRNA abundance of thirty-eight genes encoding important enzymes, ligand-dependent nuclear receptors, transcription regulators and transporters in lipid metabolism. Feeding growing male mice diets supplemented with 0.3% trans-10, cis-12-CLA for 6 weeks significantly increased the expression of hepatic genes involved in fatty acid uptake (Cd36), TG synthesis (Acaca, Gpam and Scd), lipid droplet formation (Plin2), VLDL assembling/secretion (Mttp and Cideb), ketogenesis and utilization (Hmgcs2 and Bdh1), fatty acid oxidation (Acox1), glyceroneogenesis (Pck1 and Pdk4). The results suggest that the increased liver weight in response to trans-10, cis-12-CLA may be due to increased fatty acid uptake, stimulated TG synthesis, enhanced formation of lipid droplets as well as insufficiently induced VLDL assembly/secretion mechanism in liver. We provided new evidences to advance in the knowledge of the effect of this supplement in a relatively low amount on hepatic gene expression in growing male mice. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2011-04-29T17:04:18Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Li_Jing.docx: 2056751 bytes, checksum: 4a050dd9069c2cc3bca64c68fab614d9 (MD5) Li_Jing.pdf: 1805956 bytes, checksum: 3c8bceedb69bb3c622f72010ce0be556 (MD5) Made available in DSpace on 2011-05-25T14:50:57Z (GMT). No. of bitstreams: 3 Li_Jing.pdf: 1574797 bytes, checksum: 51ddeeb756bb12665ab681dbecb2adee (MD5) license.txt: 4056 bytes, checksum: f93e65fa9d48c9ccc566eaf262ca7e3b (MD5) Li_Jing.docx: 2069080 bytes, checksum: 50d95ac3ef4e27674397004a0f6b9be0 (MD5) http://hdl.handle.net/2142/24310 en Copyright 2011 Jing Li Conjugated linoleic acid hepatic steatosis Hepatic gene networks due to the trans-10, cis-12-conjugated linoleic acid supplementation in growing male mice Thesis M.S. MS:Nutritional Sciences -UIUC 10KS0191MS Nutritional Sciences 1971 Nutritional Sciences 0191 University of Illinois at Urbana-Champaign