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oai:www.ideals.illinois.edu:2142/24412 2023-07-10 col_2142_5131 col_2142_11615 com_2142_5130 com_2142_9130 com_2142_8903

Schroeder, Charles M. Brockman, Christopher A. 2011-05-25T14:40:33Z 2011-05-25T14:40:33Z 2013-05-26T10:00:25Z 2011-05-25T14:40:33Z 2011-05 The study of polymer dynamics has been an active area of research for many years. For nearly two decades, fluorescently-labeled double stranded DNA (dsDNA) has been the model system for studying single molecule polymer dynamics in non-equilibrium conditions. However, dsDNA is a semiflexible polymer with markedly different local molecular properties compared to flexible polymer chains, such as synthetic organic polymers. In this work, we developed a new model system for single molecule studies of flexible polymers based on single stranded DNA (ssDNA). Using a biochemical synthesis scheme called rolling circle replication, long strands (>25 µm) of ssDNA containing “designer sequences” have been generated to prevent intramolecular base pairing. Polymer chains are synthesized with amine modified bases incorporated along the backbone to facilitate labeling with fluorescent dyes. As proof-of-principle demonstration of this new chemical platform, we use epifluorescence microscopy to image individual ssDNA molecules stretching in an extensional flow generated inside a microfluidic device. It is anticipated that the model polymer system presented here will serve to promote further investigations of flexible polymer dynamics at the molecular level. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2011-04-28T17:28:32Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Brockman_Christopher.docx: 2771208 bytes, checksum: 25b2aa8f45d07c262ad51ad950265a97 (MD5) Brockman_Christopher.pdf: 3520987 bytes, checksum: 3e5e20446f38d7012be439c9c03322e3 (MD5) Made available in DSpace on 2011-05-25T14:40:33Z (GMT). No. of bitstreams: 2 Brockman_Christopher.pdf: 3521508 bytes, checksum: 8e830b5f3d8e41f9881c118bce1180a8 (MD5) license.txt: 4070 bytes, checksum: 6f3492e72a06aecd72663582cd111ca6 (MD5) Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by William Ingram (wingram2@illinois.edu) on 2011-05-25T14:42:04Z Item is restricted until 2013-05-25T14:41:28Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2013-05-26T10:00:25Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Chemical and Biomolecular Engineering (ID: 591) No. of bitstreams: 2 Brockman_Christopher.pdf: 3521508 bytes, checksum: 8e830b5f3d8e41f9881c118bce1180a8 (MD5) license.txt: 4070 bytes, checksum: 6f3492e72a06aecd72663582cd111ca6 (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2013-05-26T10:00:25Z http://hdl.handle.net/2142/24412 en Copyright 2011 Christopher A. Brockman single molecule polymer dynamics rolling circle replication fluorescence microscopy Single stranded DNA: A new model system for single molecule polymer dynamics Chemical & Biomolecular Engr 1687 Chemical Engineering 0300 University of Illinois at Urbana-Champaign Thesis M.S. PHD:Chemical Engineering -UIUC 10KS0300PHD