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oai:www.ideals.illinois.edu:2142/24487 2023-07-10 col_2142_5131 col_2142_11615 com_2142_5130 com_2142_9130 com_2142_8903

Micropatterning neutrophil chemotaxis Kenis, Paul J.A. Kenis, Paul J.A. Rao, Christopher V. Wang, Fei Pack, Daniel W. Kapoor, Ashish 2011-05-25T14:23:00Z 2011-05-25T14:23:00Z 2013-05-26T10:00:19Z 2011-05-25T14:23:00Z 2011-05 Microscale systems have been finding increasing and important applications over the recent years in tissue engineering and biological studies. Recent advances in microfabrication technologies have enabled these systems to precisely control the cellular microenvironments and investigate cell behavior in vitro. This work aims at integration of advances in life sciences and microfabrication techniques to address fundamental biological questions underlying cellular behavior. We specifically focus on two applications related to human health (1) using micropatterned surfaces for tendon regeneration, and (2) using microfluidic platforms for neutrophil chemotaxis. Both of these applications are of crucial importance in modern biological and medical advancement. We have developed micropatterned platforms for systematic analysis of tenocyte behavior on topographical surfaces. These studies investigate the role of microtopographical features on the cytomorphology, alignment, proliferation, extra cellular matrix synthesis, and gene expression of tenocytes. We have also demonstrated microfluidic platforms as powerful tools for quantitative analysis of intracellular and extracellular events in neutrophil chemotaxis. Using fluorescence-labeled cells, these microfluidic platforms are used to investigate the localization of key regulatory molecules, Actin and PHAKT involved in signaling pathways during chemotaxis. Furthermore, we employ these platforms to understand macroscale neutrophil response to multiple chemoattractant gradients. The analysis of cell migration behavior in response to single and opposing gradients of chemoattractants have begun to elucidate how neutrophils integrate and prioritize multiple chemotactic cues. These results provide insight into complex cellular behavior and involved mechanisms and should aid in the design of novel therapeutic strategies to treat human health disorders. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2011-04-17T20:34:53Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Kapoor_Ashish.pdf: 4943688 bytes, checksum: fc04bb3a035b0a3eafd86bb059da899c (MD5) Made available in DSpace on 2011-05-25T14:23:00Z (GMT). No. of bitstreams: 2 Kapoor_Ashish.pdf: 4943693 bytes, checksum: ae8937489f26fcad7c6ecfc48fc063d4 (MD5) license.txt: 4063 bytes, checksum: 82cd8faf0285daf4772a8bbe018c6913 (MD5) Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2011-05-25T14:29:59Z Item is restricted until 2013-05-25T14:29:35Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2013-05-26T10:00:19Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Chemical and Biomolecular Engineering (ID: 591) No. of bitstreams: 3 Kapoor_Ashish.pdf.txt: 301893 bytes, checksum: fe1e5b6a0d8611e73e9ba2cec90e7eae (MD5) Kapoor_Ashish.pdf: 4943693 bytes, checksum: ae8937489f26fcad7c6ecfc48fc063d4 (MD5) license.txt: 4063 bytes, checksum: 82cd8faf0285daf4772a8bbe018c6913 (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2013-05-26T10:00:19Z http://hdl.handle.net/2142/24487 en 2011 Ashish Kapoor Mirofluidics tendon regeneration Engineering cellular microenvironments for directed cell growth and migration Chemical & Biomolecular Engr 1687 Chemical Engineering 0300 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Chemical Engineering -UIUC 10KS0300PHD