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oai:www.ideals.illinois.edu:2142/26322 2023-07-10 col_2142_5131 col_2142_8888 com_2142_5130 com_2142_8887 com_2142_234

Bashir, Rashid Bashir, Rashid Lyding, Joseph W. Pop, Eric Aksimentiev, Aleksei Venkatesan, Bala Murali K. 2011-08-26T15:22:55Z 2013-08-27T10:00:22Z 2011-08-26T15:22:55Z 2011-08 Nanopore DNA analysis is an emerging technique that involves electrophoretically driving DNA molecules through a nano-scale pore in solution and monitoring the corresponding change in ionic pore current. This versatile approach permits the label-free, amplification-free analysis of charged polymers (single stranded DNA, double stranded DNA and RNA) ranging in length from single nucleotides to kilobase long genomic DNA fragments with subnanometer resolution. Recent advances in nanopores suggest that this low-cost, highly scalable technology could lend itself to the development of third generation DNA sequencing technologies, promising rapid and reliable sequencing of the human diploid genome for under $1000. Here, we report the development of versatile, nano-manufactured Al2O3 solid-state nanopores and nanopore arrays for rapid, label-free, single-molecule detection and analysis of DNA and protein. This nano-scale technology has proven to be reliable, affordable, and mass producible, and allows for integration with VLSI processes. A detailed characterization of nanopore performance in terms of electrical noise, mechanical robustness and materials analysis is provided, and the functionality of this technology in experimental DNA biophysics is explored. A framework for the application of this technology to medical diagnostics and sequencing is also presented. Specifically, studies involved the detection of DNA-protein complexes, a viable strategy in screening methylation patterns in panels of genes for early cancer detection, and the creation of lipid bilayer coated nanopore sensors, useful in creating hybrid biological/solid-state nanopores for DNA sequencing applications. The concept of a gated nanopore is also presented with preliminary results. The fabrication of this novel system has been enabled by the recent discovery of graphene, a highly versatile material with remarkable electrical and mechanical properties. Direct modulation of the nanopore conductance was observed through the application of potentials to the graphene gate. These exciting results suggest this technology could potentially be useful in slowing down or trapping a DNA molecule in the pore, thereby enabling solid-state nanopore sequencing. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2011-07-07T20:12:20Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Venkatesan_BalaMurali.pdf: 19120910 bytes, checksum: 2c7572e5cfde7340490dbf86eff03a8f (MD5) Made available in DSpace on 2011-08-26T15:22:55Z (GMT). No. of bitstreams: 2 Venkatesan_BalaMurali.pdf: 19120910 bytes, checksum: 2c7572e5cfde7340490dbf86eff03a8f (MD5) license.txt: 4065 bytes, checksum: e1c422156fc8931783e8de0a596175ad (MD5) Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by William Ingram (wingram2@illinois.edu) on 2011-08-26T15:26:00Z Item is restricted until 2013-08-26T15:25:28Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2013-08-27T10:00:22Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Electrical and Computer Engineering (ID: 446) No. of bitstreams: 2 Venkatesan_BalaMurali.pdf: 19120910 bytes, checksum: 2c7572e5cfde7340490dbf86eff03a8f (MD5) license.txt: 4065 bytes, checksum: e1c422156fc8931783e8de0a596175ad (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2013-08-27T10:00:22Z http://hdl.handle.net/2142/26322 en Copyright 2011 Bala Murali K. Venkatesan Nanopore DNA Sequencing Hybrid Biological/Solid-State Al2O3 DNA-Protein Complexes Deoxyribonucleic acid (DNA) Solid-state nanopore sensors for nucleic acid analysis Electrical & Computer Eng 1933 Electrical & Computer Engr 1200 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Electr & Computer Eng-UIUC 10KS1200PHD