2026-09-20T23:35:26Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/29501 2023-07-10 col_2142_11615 col_2142_5131 com_2142_9130 com_2142_8903 com_2142_5130

Zhao, Huimin Luo, Yunzi 2012-02-01T00:49:35Z 2014-02-01T11:00:31Z 2011-12 2012-02-01T00:49:35Z 2011-12 Microorganisms and plants have evolved to produce a myriad array of natural products that are of biomedical importance. One group of valuable natural products is polyketides, which are known to be antiviral, antibacterial, and anticancer. In my first project, I attempted to develop an efficient strategy for cloning and characterizing Type III PKS genes that are likely responsible for the synthesis of novel natural products from Eucalyptus species. Nested degenerate primers were designed and a total of 94 clones and 27 clones were sequenced from the cDNA libraries created from the leaves of E. camaldulensis and E. robusta, respectively. Five unique putative Type III PKSs genes were identified. Two full-length genes were cloned and the biochemical characterization of these genes indicated their functions. In my second project, I attempted to develop a synthetic biology approach for natural product discovery. Investigation into the unknown compounds synthesized by actinomycetes may lead to the discovery of novel chemotherapeutic agents. As proof of concept, one cryptic pathway from Streptomyces griseus, containing a PKS/NRPS hybrid gene, was chosen as a model system. To decipher this cluster, I have been attempting to develop a new strategy based on our recently developed “DNA assembler” method. By using this approach, the gene cluster was modified by adding different constitutive promoters in front of each gene. RT-qPCR was employed to track expression on the transcription level. HPLC and LC-MS were used to detect the products. A potential product was detected and further characterization is in progress. Item withdrawn by Katherine Eriksen (eriksen3@illinois.edu) on 2011-12-01T21:54:10Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Luo_Yunzi.docx: 2673054 bytes, checksum: a48c792cc29496b5b6884b8a85e56c15 (MD5) Luo_Yunzi.pdf: 2148075 bytes, checksum: 545056cd2f3ac00533f01ade4090e81f (MD5) Made available in DSpace on 2012-02-01T00:49:35Z (GMT). No. of bitstreams: 2 Luo_Yunzi.pdf: 2148055 bytes, checksum: f43f79474554cd6c1c71c64f4bc48ece (MD5) license.txt: 4056 bytes, checksum: bbb08ae428c35993c7ae2bae0aaa9252 (MD5) Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by William Ingram (wingram2@illinois.edu) on 2012-02-01T00:50:44Z Item is restricted until 2014-02-01T00:50:07Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2014-02-01T11:00:31Z Item was in collections: Dissertations and Theses - Chemical and Biomolecular Engineering (ID: 591) Graduate Theses and Dissertations at Illinois (ID: 204) No. of bitstreams: 2 Luo_Yunzi.pdf: 2148055 bytes, checksum: f43f79474554cd6c1c71c64f4bc48ece (MD5) license.txt: 4056 bytes, checksum: bbb08ae428c35993c7ae2bae0aaa9252 (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2014-02-01T11:00:31Z http://hdl.handle.net/2142/29501 en Copyright 2011 Yunzi Luo bioactive plants polyketides type III PKS synthetic biology DNA assembler Polyketide synthases (PKS) Discovering novel natural products from bioactive plants and bacteria Dissertation / Thesis text Chemical & Biomolecular Engr 1687 Chemical Engineering 0300 University of Illinois at Urbana-Champaign Thesis M.S. PHD:Chemical Engineering -UIUC 10KS0300PHD