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oai:www.ideals.illinois.edu:2142/30972 2023-07-10 col_2142_5131 col_2142_8859 com_2142_5130 com_2142_8858 com_2142_234

Ha, Taekjip Chemla, Yann R. Ha, Taekjip Oono, Yoshitsugu Stack, John D. Jena, Prakrit 2012-05-22T00:19:16Z 2012-05-22T00:19:16Z 2012-05 2012-05-22T00:19:16Z 2012-05 Single molecule microscopy has been extensively used in the past decade to study individual biomolecules with excellent spatial and temporal resolution. Meanwhile, characterization of nanomaterials and their development towards a variety of biological applications has progressed rapidly. Despite the comparable size of nanoparticles and biomolecules, no microscopy platform and technique currently exists to study the interactions of single biomolecules with nanoparticles. Here, we have successfully developed a set of experimental tools to study the interactions of DNA and proteins on a nanomaterial surface. We observe nucleic acid‐encapsulated carbon nanotubes by using fluorophore‐labeled complementary DNA to explore the sequence‐specific affinity of DNA to the nano‐surface. Our results demonstrate cooperative exfoliation of the oligonucleotides from the nanomaterial surface and sequence‐dependent bioavailability of nanotube‐adsorbed DNA for hybridization. The platform is employed in conjunction with a super resolution algorithm to pinpoint sites of DNA hybridization along the length of the nanotube. The ability of a microfluidic channel to easily exchange solution is used to study specific and non‐specific nuclease activity on nanotube‐adsorbed DNA. Protein function is mapped to local DNA topology on the nanomaterial and distance‐dependent arrest of protein activity is observed, resulting in a nanotube‐induced arrest of 60% protein activity within 1 nm from the nanoparticle. Accessibility of different points of contact between the DNA and nanotube are assayed for nuclease resistance and range from 5% to 50%. Item withdrawn by Alexis Thompson (athmpsn1@illinois.edu) on 2012-04-19T14:55:30Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Jena_Prakrit.pdf: 5920107 bytes, checksum: c69c77373dba35dadab8714d2d1cf8c0 (MD5) Made available in DSpace on 2012-05-22T00:19:16Z (GMT). No. of bitstreams: 2 Jena_Prakrit.pdf: 5925225 bytes, checksum: 05c0c4706be4b9147350b8fb57913ef8 (MD5) license.txt: 4060 bytes, checksum: fe257e3eb78ee2b84a27e7100fd72f07 (MD5) http://hdl.handle.net/2142/30972 en Copyright 2012 Prakrit Vaibhav Jena single molecule microscopy carbon nanotubes Fluorescence resonance energy transfer (FRET) Telomeric DNA DNA-SWNT Deoxyribonucleic acid (DNA) Single molecule fluorescence microscopy of carbon nanotubes text Physics 1244 Physics 0240 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Physics -UIUC 10KS0240PHD