2026-09-21T23:07:45Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/31042 2023-07-10 col_2142_5131 col_2142_14771 com_2142_5130 com_2142_685 com_2142_397

Fahey, George C. Fahey, George C. Dilger, Ryan N. Parsons, Carl M. Tappenden, Kelly A. Faber, Trevor 2012-05-22T00:23:58Z 2012-05-22T00:23:58Z 2012-05 2012-05-22T00:23:58Z 2012-05 The objective of this research was to evaluate a novel galactoglucomannan oligosaccharide-arabinoxylan (GGMO-AX) complex for properties that could positively impact nutritional and immunological outcomes. Five studies were designed to address three major research objectives: 1) determine the structural and chemical composition of the GGMO-AX substrate and select fractions, 2) determine the hydrolytic digestibility and fermentative capacity of the GGMO-AX substrate in vitro and in vivo, and 3) determine the immunological effects of GGMO-AX in a pathogen-challenged avian model. Study 1 evaluated the structural composition of the GGMO-AX components as determined by a combination of limited hydrolysis, monosaccharide composition and linkage analysis, size exclusion fractionation and MALDITOF/ MS analysis of component GGMO, and 1D and 2D NMR techniques. Study 2 evaluated the hydrolytic digestibility, fermentative capacity, and microbiota modulating properties of GGMO-AX and four fractions of GGMO-AX. Study 3 evaluated nutritional effects and prebiotic potential of spray-dried GGMO-AX when added to canine diets and tested in a doseresponse experiment. Studies 4 and 5 determined the effects of supplemental GGMO-AX in diets with emphasis on growth performance, fermentative effects, and immune indices in an avian model challenged with an acute coccidial (Eimeria acervulina; EA) or Salmonella typhimurium (ST) infection. Results indicated that GGMOs have a degree of polymerization (DP) from 4 to 13, with the major component being DP 5-8. The structure of these oligosaccharides is a β-1,4-linked backbone of Man and Glc residues, with occasional α-1,6 branching by single galactosyl units. The GGMO-AX substrate is resistant to hydrolytic digestion, well-fermented, and positively modulates microbial populations as measured in vitro and in vivo. When chicks were challenged with EA, a strain of avian coccidiosis, and iii supplemented with select concentrations of GGMO-AX, chick performance was decreased, but GGMO-AX supplementation improved select fermentation indices and the innate intestinal immune response. During a ST infection, GGMO-AX elicited a prebiotic effect and appeared to decrease the virulence of the ST within the digestive tract, but did not limit ST intestinal colonization or shedding. Overall, GGMO-AX appears to be well fermented in vitro and in vivo and able to elicit a prebiotic effect in select animal models. Dietary GGMO-AX supplementation is able to improve the innate immune response to an EA infection and potentially decrease ST virulence. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-01-04T14:53:37Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Faber_Trevor.docx: 1825209 bytes, checksum: f4d994b2cd08ead6dcb7effe649893cb (MD5) Faber_Trevor.pdf: 1460654 bytes, checksum: cdb5dea09028670cc4139724801a88b2 (MD5) Made available in DSpace on 2012-05-22T00:23:58Z (GMT). No. of bitstreams: 3 Faber_Trevor.pdf: 1460740 bytes, checksum: 9465d148135a0a1473124df13e1ad3af (MD5) Faber_Trevor.docx: 1825209 bytes, checksum: f4d994b2cd08ead6dcb7effe649893cb (MD5) license.txt: 4060 bytes, checksum: fe50d21a7849bc13b752268097bdee68 (MD5) http://hdl.handle.net/2142/31042 en Copyright 2012 Trevor Austin Faber Galactoglucomannan oligosaccharide Dietary Fiber Eimeria acervulina Salmonella typhimurium In vitro fermentation Prebiotic Nutritional and immunological outcomes as affected by a novel carbohydrate complex composed of galactoglucomannan oligosaccharides and arabinoxylan text Animal Sciences 1538 Animal Sciences 0002 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Animal Sciences -UIUC 10KS0002PHD