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oai:www.ideals.illinois.edu:2142/32058 2023-07-10 col_2142_5131 col_2142_14789 com_2142_5130 com_2142_14788 com_2142_8903

Kenis, Paul J.A. Leckband, Deborah E. Kenis, Paul J.A. Gaskins, H. Rex Bailey, Ryan C. Zhang, Jieqian 2012-06-27T21:30:54Z 2014-06-28T10:00:28Z 2012-05 2012-06-27T21:30:54Z 2012-05 Microfluidics manipulates, processes, and controls small volumes of fluids in channels or compartments with cross-sectional dimensions on the order of 10-100 µm. By integrating functional components such as channels, actuators, and sensors, and by leveraging characteristic physical phenomena occurring at the microscale, microfluidics brings microfabrication, chemistry and physics together to develop new experimental paradigm for broad applications, including the study of biological problems. Indeed, microfluidics operates at a scale that is particularly suitable for biological sample analysis, which provides great opportunities for technology innovation in bioanalytical assays and biomedical applications such as point-of-care devices. Challenges due to the complexity of the biological system can be overcome by introducing new engineering elements and performing systematic device optimization. In this work, a microfluidic immunoaffinity based silicon micropost device for hematopoietic stem cell enrichment is discussed, which demonstrates how innovations in microfluidics can revolutionize current clinical therapies. Appropriate surface chemistry for robust immobilization of proteins on the surface of different materials and topography was developed. Silicon chips with three-dimensional features, arrays of microposts in a wide range of arrangements, were designed. The fluid dynamics in the various micropost arrays was modeled and optimized to meet specific needs of cell enrichment for clinical application. The optimized three-dimensional micropost devices achieve hematopoietic stem cell enrichment at a level that outperforms all current technologies and successfully meets the requirements for clinical transplantation applications. Item withdrawn by Rebecca Bryant (rabryant@illinois.edu) on 2012-01-31T14:24:32Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Zhang_Jieqian.pdf: 14627286 bytes, checksum: 9584f9ad71fb6f7f93e57d99bed30c40 (MD5) Made available in DSpace on 2012-06-27T21:30:54Z (GMT). No. of bitstreams: 2 Zhang_Jieqian.pdf: 15685604 bytes, checksum: 64322330ac59d37a39cb660cbeb712dd (MD5) license.txt: 4063 bytes, checksum: 75a61ed828ccc30ac3df885ffd43ec1c (MD5) Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2012-06-27T21:32:46Z Item is restricted until 2014-06-27T21:32:23Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2014-06-28T10:00:28Z Item was in collections: Graduate Theses and Dissertations at Illinois (ID: 204) Dissertations and Theses - Chemistry (ID: 676) No. of bitstreams: 2 Zhang_Jieqian.pdf: 15685604 bytes, checksum: 64322330ac59d37a39cb660cbeb712dd (MD5) license.txt: 4063 bytes, checksum: 75a61ed828ccc30ac3df885ffd43ec1c (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2014-06-28T10:00:28Z http://hdl.handle.net/2142/32058 en Copyright 2012 Jieqian Zhang Microfluidics cell enrichment hematopoietic stem cell A microfluidic platform for hematopoietic stem cell separation text Chemistry 1413 Chemistry 0335 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Chemistry -UIUC 10KS0335PHD