2026-09-21T16:19:02Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/34263 2023-07-10 col_2142_5131 col_2142_16308 com_2142_5130 com_2142_16307 com_2142_8913 com_2142_8903

Robinson, Gene E. Hudson, Matthew E. Berenbaum, May R. Yu, Johnny 2012-09-18T21:08:31Z 2012-09-18T21:08:31Z 2012-08 2012-09-18T21:08:31Z 2012-08 At least eighteen different picornaviruses can infect honey bees (Apis mellifera). The combination of the virulence of these pathogens as well as their transmissibility through Varroa destructor, an ectoparasitic mite, can cause severe declines in honey bee populations. Polyadenylated (polyA) rRNA has been associated with honey bees infected with viruses in past studies. In bacteria, yeast, and some mammals, polyA rRNA serves as a molecular marker for the degradation of rRNA. Honey bees may be utilizing polyA rRNA in the same fashion to degrade cells that are infected with picornaviruses. In this thesis, I attempted to correlate viral infection and polyA rRNA using qRT-PCR and RNA-seq. Five polyA regions were identified with RNA-seq. One region (28S 2070-2130) was weakly associated with viral infection. I used quantitative RT-PCR to more accurately measure the 28S 2070-2130 region and again found a weak association between viral infection and polyA rRNA. Also, significantly more polyA rRNA was found in the abdomen than in the head. The high expression levels of polyA rRNA in the abdomen may be a consequence of picornaviruses that infect mostly the abdomen. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-07-17T21:11:15Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Yu_Johnny.docx: 1193912 bytes, checksum: 03cc9367608cc70a7f020ec46b4dc4f6 (MD5) Yu_Johnny.pdf: 946512 bytes, checksum: 09fbc097882987b2b648dfe5c75c51e2 (MD5) Made available in DSpace on 2012-09-18T21:08:31Z (GMT). No. of bitstreams: 3 Yu_Johnny.pdf: 946713 bytes, checksum: 7f155a1e8981c5d759119183d9445b23 (MD5) license.txt: 4055 bytes, checksum: 363ea6b4b303176b19ed42f81c104834 (MD5) Yu_Johnny.docx: 1200937 bytes, checksum: d585c384234aafadb93bcf235e6c56e8 (MD5) http://hdl.handle.net/2142/34263 en Copyright 2012 Johnny Yu Apis mellifera virus immunity picornavirus ribosomal RNA Polyadenylation of ribosomal RNA in response to picornavirus infection in honey bees (Apis mellifera) Entomology 1361 Entomology 0324 University of Illinois at Urbana-Champaign Thesis M.S. MS:Entomology -UIUC 10KS0324MS