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Baranger, Anne M. Baranger, Anne M. Katzenellenbogen, John A. Hergenrother, Paul J. Zhao, Huimin Baek, Jung-Un 2012-09-18T21:13:39Z 2012-09-18T21:13:39Z 2012-08 2012-09-18T21:13:39Z 2012-08 The RNA recognition motif (RRM) is the most abundant RNA binding domain that is found in all organisms. RRM-containing proteins participate in most steps of gene expression, including translation, splicing, modification and transport of RNA. This dissertation aims to help understand the interactions of the RNA recognition motif and RNA by developing a small molecule modulator of the interaction and analyzing the kinetics of dissociation. The first chapter gives an introduction to the function and structural characteristics of RNA binding proteins, RNA recognition motifs and two RRM proteins, U1A and Sex lethal protein. Chapter 2 describes the identification and analysis of three small molecules that disrupt two different RRM-RNA complexes, Sex lethal protein-tra RNA and U1A-SL2 RNA. The research discussed in chapter 3 focus on the role of positively charged residues in the U1A protein and SL2 RNA complex dissociation process. Analysis of kinetics data obtained by temperature jump and stopped-flow experiments showed that the location of the electrostatic interaction controls the rate of different steps in the complex dissociation pathway. Chapter 4 is a description of a simple and rapid method to detect RNA splice variants using biarsenical dyes and split tetracysteine moieties, which may accelerate biochemical studies of alternative splicing and identification of factors that modulate RNA splicing. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-06-26T14:38:19Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Baek_Jung-Un.pdf: 35167734 bytes, checksum: 6e54930ffdfa8d609a9c52a77ceadb7e (MD5) Made available in DSpace on 2012-09-18T21:13:39Z (GMT). No. of bitstreams: 2 Baek_Jung-Un.pdf: 35167890 bytes, checksum: f2be5070f53a2795a8800f6ec79e608d (MD5) license.txt: 4057 bytes, checksum: 90fb15288b738b48e32cb3cd76e5f93d (MD5) http://hdl.handle.net/2142/34369 en Copyright 2012 Jung-Un Baek RNA binding protein Small molecule RNA recognition motif Kinetics Protein-RNA interaction Investigation of the interactions in RNA recognition motif-RNA complexes: small molecule inhibitors and kinetics of dissociation Chemistry 1413 Chemistry 0335 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Chemistry -UIUC 10KS0335PHD