2026-09-23T21:42:01Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/34398 2023-07-11 col_2142_5131 col_2142_16508 com_2142_5130 com_2142_16507 com_2142_14793 com_2142_8903

Gillette, Martha U. Gillette, Martha U. Chung, Hee Jung Cox, Charles L. Gillette, Rhanor Wang, Yingxiao Wang, Tongfei 2012-09-18T21:15:02Z 2012-09-18T21:15:02Z 2012-08 2012-09-18T21:15:02Z 2012-08 Daily rhythms of mammalian physiology, metabolism, and behavior parallel the day-night cycle. They are driven by the central circadian clock in the brain, the suprachiasmatic nucleus (SCN), where a genetic oscillator plays an essential role. Clock-gene transcription/translation is sensitive to metabolic (redox) change; however, energetic cycles manifest as circadian rhythms in protein oxidation have been reported in anucleate cells, where no transcription occurs. Whether the brain clock expresses redox cycles and how such metabolic oscillations might affect neuronal physiology are unknown. Here we show a self-sustained circadian rhythm of SCN redox state that requires the molecular clockwork. The redox oscillation determines the excitability of SCN neurons through a non-transcriptional mechanism: alterations in redox state rapidly (< 2 min) reverse membrane polarization of SCN neurons via changes in multiple K+ channels. The redox regulation of neuronal excitability gates the SCN sensitivity and response to entraining signals of light, by modulating Ca2+ signaling in response to excitatory neurotransmission, targeting on a ryanodine receptor–dependent intracellular Ca2+ store. Our study provides a novel pathway for the metabolic oscillator to engage in the organization with the central circadian clock; it couples the molecular clockwork and cellular energetics with membrane physiology, suggesting a basis for dynamic regulation of SCN excitability that is closely tied to metabolism. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-07-03T18:31:26Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Wang_Tongfei.pdf: 1349438 bytes, checksum: 788e7a2202d1824b2a5e5f49e6ebb236 (MD5) Made available in DSpace on 2012-09-18T21:15:02Z (GMT). No. of bitstreams: 2 Wang_Tongfei.pdf: 1349706 bytes, checksum: 3f0d36b9bdc15dc024c56efb25f00096 (MD5) license.txt: 4061 bytes, checksum: e7e995186be0b0ef94869bcd79f8f71c (MD5) http://hdl.handle.net/2142/34398 en Copyright 2012 Tongfei Wang circadian rhythm suprachiasmatic nucleus (SCN) redox excitability Ca2+ phase shift Circadian rhythms of redox state and regulation of neuronal excitability in suprachiasmatic nucleus of rodents Molecular & Integrative Physl 1604 Molecular & Integrative Physi 0325 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Mole & Integrat Phys -UIUC 10KS0325PHD