2026-09-23T02:37:44Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/34568 2023-07-11 col_2142_14795 col_2142_5131 com_2142_14794 com_2142_14793 com_2142_8903 com_2142_5130

Hergenrother, Paul J. Knowlton, Aaron 2012-09-18T21:25:58Z 2012-09-18T21:25:58Z 2014-09-18T10:00:37Z 2012-08 2012-09-18T21:25:58Z 2012-08 Normal miRNA production is essential for regulating protein expression and cellular fate. Misregulation of miRNA production is emerging as a key factor in the development of many disease states, including most forms cancer. A global downregulation of miRNA processing is common among cancers and has been shown to drive tumorogenesis. This global downregulation of miRNA biogenesis is caused by the disruption of key enzymes in the miRNA biogenesis pathway like Dicer, TAR RNA-binding protein (TRBP) and Drosha. Herein, the enhancement of miRNA processing as a therapeutic strategy is explored. Developing small molecule or peptide based therapeutics that mimic TRBP, an endogenous activator of Dicer, to enhance miRNA processing in cancers with reduced miRNA production may lead to safe and effective treatments for a number of cancer patients. Identification of direct activators of Dicer will be explored using in vitro kinetic assays with fluorescent probes. Expression of Dicer, TRBP and a peptide-mapping library of TRBP are also described. Since miRNA biogenesis is such a critical pathway in tumorogenesis, activation of this pathway may prove to be an effective target in the battle against cancer. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-07-20T15:29:10Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Knowlton_Aaron.pdf: 4690869 bytes, checksum: eb52242688f8f2b30c9c0684faf84f1c (MD5) Made available in DSpace on 2012-09-18T21:25:58Z (GMT). No. of bitstreams: 2 Knowlton_Aaron.pdf: 4710956 bytes, checksum: c58723c8819f68d4257b574051a93e47 (MD5) license.txt: 4064 bytes, checksum: 799aa2d82dc354c1aab31a509fcc887b (MD5) Restriction data tranferred 2014-07-01T11:35:51-05:00 Original Data Group with Access Administrator Release Date: 2014-09-18 16:27:16 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Item marked as restricted to the 'Administrator' Group (id=1) by Seth Robbins (srobbins@illinois.edu) on 2012-09-18T21:27:32Z Item is restricted until 2014-09-18T21:27:16Z Limited Restriction Lifted for Item 34842 on 2014-09-18T10:00:37Z. http://hdl.handle.net/2142/34568 en Copyright 2012 Aaron Knowlton Dicer transactivating response RNA-binding protein (TRBP) Dicer1 transactivating response RNA-binding protein 2 (tarbp2) micro ribonucleic acid (miRNA) cancer Hereditary nonpolyposis colorectal cancer (HNPCC) aptamer Malachite green Enhancement of Dicer as a personalized anticancer strategy Biochemistry 1438 Biochemistry 0318 University of Illinois at Urbana-Champaign Thesis M.S. PHD:Biochemistry -UIUC 10KS0318PHD