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oai:www.ideals.illinois.edu:2142/35217 2023-07-11 col_2142_8859 col_2142_5131 com_2142_8858 com_2142_234 com_2142_5130

Clegg, Robert M. Redford, Glen Ivan 2012-11-14T16:43:28Z 2012-11-14T16:43:28Z 10000-01-01 2005-12 A fast, full-field fluorescence lifetime imaging system based on the homodyne technique was built and used for several applications. Also contained herein is the basic theory and background for fluorescence lifetime imaging, as well as data analysis of lifetime distributions. The fast, easy-to-use lifetime imager has proven useful for many applications and essential for several. Examples of applications where such an instrument is needed (and where a conventional system is inadequate) include: any system with large photo-bleaching effects, systems where the intensity changes on the timescale of the measurement, and situations that require real-time display of lifetime images. Measuring proto-porphyrin IX in cells is an example of a system where photobleaching makes it difficult to measure. Measuring the lifetime changes in dark adapted plant leaves upon exposure to light is an example of a system with complicated intensity changes. Real-time display of lifetime images is required for clinical work. This paper includes details of the hardware and software for the instrument as well as data taken with the instrument. Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-11-14T16:43:28Z No. of bitstreams: 1 Redford_Glen.pdf: 5402533 bytes, checksum: 444394cf6cce2eafee22782855255477 (MD5) Made available in DSpace on 2012-11-14T16:43:28Z (GMT). No. of bitstreams: 1 Redford_Glen.pdf: 5402533 bytes, checksum: 444394cf6cce2eafee22782855255477 (MD5) Previous issue date: 2005-12 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Meng Tao (mengtao2@illinois.edu) on 2012-11-14T16:43:29Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:12:13-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: Post 1923. No authorization form. Post 1923. No authorization form. U of I Only 5438321 http://hdl.handle.net/2142/35217 en ©2005 Glen Ivan Redford Fluorescence Lifetimes Imaging Fluorescence signals Chebyshev polynomials Full-field homodyne Fast Fluorescence Lifetime Imaging Using a Full-Field Homodyne System with Applications in Biology Dissertation / Thesis text Physics Physics University of Illinois at Urbana-Champaign Dissertation Ph.D.