2026-09-23T11:43:51Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/44137 2023-07-11 col_2142_5131 col_2142_25441 com_2142_5130 com_2142_25440 com_2142_14833

Lau, Gee W. Jeffries, Jayme 2013-05-24T21:52:22Z 2013-05-24T21:52:22Z 2015-09-26T10:01:20Z 2013-05 2013-05-24T21:52:22Z 2013-05 Cystic fibrosis (CF) patients battle life-long pulmonary infections with the respiratory pathogen Pseudomonas aeruginosa (PA). A favorable niche for PA growth is provided by an overabundance of mucus in CF airways. When compared to that of non-CF individuals, mucus of CF airways has been found to be enriched in sialic acid, especially in the form of the tetra carbohydrate moiety sialyl-Lewisx, which is a preferred binding receptor for PA. Furthermore, sialyl-Lewisx levels have previously been shown to directly correlate with infection levels in CF patients. In this study, we examined the ability of PA virulence factors pyocyanin (PCN), LPS, flagella and alginate to modulate the levels of sialyl-Lewisx modifications on airway mucins in a mouse model and in in vitro cell culture. We found PCN to be a potent inducer of sialyl-Lewisx in both mouse airways and in the cultured airway epithelial cells. PCN increases the expression of ST3Gal-IV and C2/4GnT, two enzymes responsible for the stepwise synthesis of sialyl-Lewisx through a TNF-α dependent phosphoinositol-specific phospholipase C (PI-PLC) pathway. Importantly, PA is better able to bind airway epithelial cells previously exposed to PCN. These results suggest that PA secretes PCN to induce a favorable environment for chronic colonization of CF lungs by increasing the glycosylation of airway mucins with sialyl-Lewisx. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-03-21T13:55:11Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Jeffries_Jayme.pdf: 1611738 bytes, checksum: 3d6cc6c98e780d0a9bae3a2b5c457c80 (MD5) Jeffries_Jayme.pdf: 1601149 bytes, checksum: 44e17db5970977cca6d9b088a0bf09c3 (MD5) Made available in DSpace on 2013-05-24T21:52:22Z (GMT). No. of bitstreams: 2 Jayme_Jeffries.pdf: 1601149 bytes, checksum: 44e17db5970977cca6d9b088a0bf09c3 (MD5) license.txt: 4064 bytes, checksum: 3970d9a5a0975e23587a2eba87a98d3d (MD5) Restriction data tranferred 2014-07-01T11:11:22-05:00 Original Data Group with Access Administrator Release Date: 2015-09-26 10:35:10 UTC Reason: Graduate College approved closed access (OA after 2 years) via email on 09/25/2013 sshreeve Item marked as restricted to the 'Administrator' Group (id=1) by Sarah Shreeves (sshreeve@illinois.edu) on 2013-09-26T15:35:11Z Item is restricted until 2015-09-26T15:35:10Z Limited Restriction Lifted for Item 44110 on 2015-09-26T10:01:20Z. http://hdl.handle.net/2142/44137 en Copyright 2013 Jayme Jeffries sialyl-Lewisx pyocyanin mucin glycoprotein cystic fibrosis Pseudomonas aeruginosa Modulation of airway mucin glycosylation by Pseudomonas aeruginosa pyocyanin text Pathobiology 1282 VMS - Veterinary Pathobiology 0361 University of Illinois at Urbana-Champaign Thesis M.S. MS:VMS-Vet Pathobiology -UIUC 10KS0361MS