2026-09-19T14:19:59Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/44261 2023-07-11 col_2142_5131 col_2142_14828 com_2142_5130 com_2142_14827 com_2142_14793 com_2142_8903

copy-number variations Single molecule-High Resolution Imaging with Photobleaching (SHRImP) High-Resolution Co-localization (SHREC) Deoxyribonucleic Acid (DNA) Selvin, Paul R. Schroeder, Charles M. Selvin, Paul R. Nayfeh, Munir H. Myong, Su-A Kuhlman, Thomas E. Baday, Murat 2013-05-24T22:05:51Z 2013-05-24T22:05:51Z 2013-05 2013-05-24T22:05:51Z 2013-05 Many types of diseases including cancer and autism are associated with copy-number variations in the genome. Most of these variations could not be identified with existing sequencing and optical DNA mapping methods. We have developed Multi-color Super-resolution technique, with potential for high throughput and low cost, which can allow us to recognize more of these variations. Our technique has made 10–fold improvement in the resolution of optical DNA mapping. Using a 180 kb BAC clone as a model system, we resolved dense patterns from 108 fluorescent labels of two different colors representing two different sequence-motifs. Overall, a detailed DNA map with 100 bp resolution was achieved, which has the potential to reveal detailed information about genetic variance and to facilitate medical diagnosis of genetic disease. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-04-15T12:40:29Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Baday_Murat.docx: 3842481 bytes, checksum: 618bb22853224e94fbfb51c9630e2540 (MD5) Baday_Murat.pdf: 3348473 bytes, checksum: 1eb0d9cd9d7f38432cb05c817650509f (MD5) Made available in DSpace on 2013-05-24T22:05:51Z (GMT). No. of bitstreams: 3 Murat_Baday.pdf: 3348473 bytes, checksum: 1eb0d9cd9d7f38432cb05c817650509f (MD5) Baday_Murat.docx: 3842481 bytes, checksum: 618bb22853224e94fbfb51c9630e2540 (MD5) license.txt: 4060 bytes, checksum: 473d000db6cc9d9567f77ecfb44fa071 (MD5) http://hdl.handle.net/2142/44261 en Copyright 2013 Murat Baday Super-resolution optical DNA mapping genomic variations High resolution optical DNA mapping text School of Molecular & Cell Bio 1415 Biophysics & Computnl Biology 0319 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Biophys&Computnl Bio -UIUC 10KS0319PHD