2026-09-21T13:19:16Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/44269 2023-07-11 col_2142_14855 col_2142_5131 com_2142_14854 com_2142_14793 com_2142_8903 com_2142_5130

Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-04-12T12:21:39Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Rhine_Jonathan.doc: 7346688 bytes, checksum: 7d38fb634a2f40d92ffce5dde98479c0 (MD5) Rhine_Jonathan.pdf: 4578816 bytes, checksum: 2ccc53b0ef859f93bdf4472218bfe547 (MD5) Williams, Benjamin Bellini, Michel Williams, Benjamin Newmark, Phillip A. Cox, Lee C. Smith-Bolton, Rachel Rhine, Jonathan 2013-05-24T22:06:07Z 2013-05-24T22:06:07Z 2013-05 2013-05-24T22:06:07Z 2013-05 Forces generated by body wall muscles of Caenorhabditis elegans must be transmitted across the worm’s thin hypodermis or “skin” to the cuticle during locomotion. Fibrous organelles (FOs), structures analogous to vertebrate hemidesmosomes, link intermediate filaments to both the apical and basal membrane of the hypodermal cells and form key elements in this force transduction pathway. Here we molecularly isolate the pat-12 gene and show that it encodes a component of the fibrous organelles. The pat-12 gene was identified through the pat-12(st430) mutation, which causes the embryonic body wall muscle cells to rip free from the body wall soon after they first begin to contract during mid-embryogenesis. The pat-12 gene, which corresponds to the complex proposed gene T17H7.4, is predicted to encode 12 different isoforms. The st430 mutation alters the conserved splice acceptor site of exon 12. The PAT-12 protein localizes to hypodermal cells in wild-type embryos, but is absent in st430 homozygotes. Here, we show that two of the four isoforms that normally include exon 12 localize to circumferential hypodermal bands characteristic of fibrous organelle components. Furthermore, we propose that PAT-12 forms part of a linkage between the fibrous organelles and the circumferential actin bands (CFB). Made available in DSpace on 2013-05-24T22:06:07Z (GMT). No. of bitstreams: 3 Jonathan_Rhine.pdf: 4578714 bytes, checksum: 666aeb07421fc29bd0ea401dc6bddfd8 (MD5) Rhine_Jonathan.doc: 7347712 bytes, checksum: 62270810afca01794d58abad7ff7dc69 (MD5) license.txt: 4069 bytes, checksum: 8b5d7945e371b6c8cb89b7f44eb3789e (MD5) http://hdl.handle.net/2142/44269 en Copyright 2013 Jonathan Rhine pat-12 elegans hemidesmosomes integrin hypodermis fibrous organelle Pat-12, an atypical Pat protein involved in fibrous organelles in C. elegans text Cell & Developmental Biology 1584 Cell and Developmental Biology 4094 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Cell&Develpmntl Biol -UIUC 10KS4094PHD