2026-09-21T15:46:36Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/44342 2023-07-11 col_2142_5131 col_2142_14828 com_2142_5130 com_2142_14827 com_2142_14793 com_2142_8903

Wang, Yingxiao Wang, Yingxiao Granick, Steve Toussaint, Kimani C. Gennis, Robert B. Eichorst, John 2013-05-24T22:08:23Z 2013-05-24T22:08:23Z 2013-05 2013-05-24T22:08:23Z 2013-05 This dissertation describes the process of optimizing a Fluorescence Lifetime Imaging Microscopy (FLIM) system in order to observe the dynamics of enzymes in live cancer cells. The enzyme studied throughout this research is Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) which is a membrane-bound protein principally responsible for degrading extra-cellular matrix (ECM) proteins in the local environment of a migrating cell. However, MT1-MMP has an intricate role in the regulation of the cell’s migration separate from its simple proteolytic functions. In addition, the increased expression of MT1-MMP has been positively correlated with the invasive potential of tumor cells. In spite of the importance of MT1-MMP in understanding a cancer cell’s decision making as it leaves a tumor, very few reports have quantitatively studied the activity of this enzyme in live cells. Even fewer reports have examined the spatiotemporal activity of MT1-MMP in live cells cultured in 3-dimensional settings such as matrices of ECM proteins. These 3-dimensional settings can parallel the environment encountered by metastasizing cells in tissues. Studying live cells in 3-dimensional matrices is crucial for biologically relevant investigations. A cell’s morphology and migratory behavior can vary significantly when comparisons are made between cells cultured on two dimensional substrates and those cultured in 3-dimensional matrices. The purpose of this project was to understand the coordinated functions of MT1-MMP as live cancer cells interact with and move through a 3-dimensional matrix of ECM proteins. Specifically, we are ultimately interested in the spatiotemporal activation patterns of MT1-MMP in live cancer cells in order to build a quantitative (systems-level) model describing MT1-MMP’s role in the cell’s decision making as it is leaves a tumor site. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-03-26T13:44:05Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Eichorst_John.pdf: 2885097 bytes, checksum: b2fcf828012c6e764b7e9e046003ee6e (MD5) Made available in DSpace on 2013-05-24T22:08:23Z (GMT). No. of bitstreams: 3 John_Eichorst.pdf: 6178714 bytes, checksum: 22860f7c913d84de3baf822399efea05 (MD5) license.txt: 4063 bytes, checksum: 30cea4fcb0d86f07c16c63f92691bc59 (MD5) 1_license.txt: 4063 bytes, checksum: 150f11a70360e17e8f9ba95ce2e9dcaf (MD5) http://hdl.handle.net/2142/44342 en Copyright 2013 John Eichorst Fluorescence Lifetime Imaging Micorscopy (FLIM) Cancer Fluorescent Proteins Metastasis Enzymatic Activity Optimization of fluorescence lifetime imaging microscopy (FLIM) for studying the activity of enzymes in live cancer cells text School of Molecular & Cell Bio 1415 Biophysics & Computnl Biology 0319 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Biophys&Computnl Bio -UIUC 10KS0319PHD