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Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-04-22T15:23:45Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Cvetkovic_Caroline.pdf: 9089391 bytes, checksum: 3bca9a127ed7823923b3518da57bc11a (MD5) Bashir, Rashid Cvetkovic, Caroline 2013-05-24T22:16:30Z 2013-05-24T22:16:30Z 2015-05-24T10:01:49Z 2013-05 2013-05-24T22:16:30Z 2013-05 Over the past decade, a new scientific discipline has emerged, integrating mechanics with biology to create complex engineered living systems. The building blocks – different cell types in an instructive environment – can be assembled in various ways to promote the emergence (or natural evolution and interaction) of the cells in a system with well-defined functionality. These functions could include sensing, information processing, protein expression, and actuation, among countless others. This Thesis presents a novel cellular system capable of actuation and fabricated using cells and hydrogels. A stereolithographic 3D printing technique (SLA) was used to create a hydrogel backbone made of a beam connecting two pillars that supports a muscle strip created from skeletal muscle cells in a fibrin-based matrix. The entire device is termed a “bio-bot,” or biological robot. Contraction of the cells within the muscle strip produced enough force to move the pillars and displace the bio-bot on a surface in a liquid medium. This Thesis is focused on the development and characterization (mechanical and biological) of the skeletal muscle-based biological actuator. The use of the SLA allowed for easy modifications of the polymerized part’s geometry and material properties. Increasing the energy dose of polymerization produced a stiffer beam that restricted bending and increased the passive tension in the muscle strip. 15-19 days after cell seeding, the bio-bots displayed spontaneous contraction that resulted in a net displacement of up to ~6 mm in 10 minutes. During this time span, a maximum velocity of over 1890 μm/min was achieved. Future plans are focused on controlling the activity of the bio-bot using optogenetics. Made available in DSpace on 2013-05-24T22:16:30Z (GMT). No. of bitstreams: 2 Caroline_Cvetkovic.pdf: 9089391 bytes, checksum: 3bca9a127ed7823923b3518da57bc11a (MD5) license.txt: 4068 bytes, checksum: 868ffd75f7907a9e7922dfffc2fefc1d (MD5) Restriction data tranferred 2014-07-01T11:36:12-05:00 Original Data Group with Access UIUC Users [automated] Release Date: 2015-05-24 17:18:31 UTC Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Seth Robbins (srobbins@illinois.edu) on 2013-05-24T22:19:00Z Item is restricted until 2015-05-24T22:18:31Z U of I Only Restriction Lifted for Item 44421 on 2015-05-24T10:01:49Z. http://hdl.handle.net/2142/44448 en Copyright 2013 Caroline Elizabeth Cvetkovic skeletal muscle bio-actuator stereolithography The development of a skeletal muscle bio-actuator using 3-D stereolithography text Bioengineering 1343 Bioengineering 0408 University of Illinois at Urbana-Champaign Thesis M.S. PHD: Bioengineering-UIUC 10KS0408PHD