2026-09-21T11:49:23Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/45574 2023-07-11 col_2142_5131 col_2142_16308 com_2142_5130 com_2142_16307 com_2142_8913 com_2142_8903

Berenbaum, May R. Noble, Katherine 2013-08-22T16:48:17Z 2013-08-22T16:48:17Z 2015-08-22T10:00:50Z 2013-08 2013-08-22T16:48:17Z 2013-08 The highly polyphagous navel orangeworm, Amyelois transitella, is a major pest of high-value tree crops such as pistachios and almonds. These non-native crops have been grown commercially in the United States for less than 150 years, and the acquisition by the navel orangeworm of these and other novel hosts may have been facilitated by its metabolic capacity to detoxify xenobiotics. Using Illumina RNA sequencing, I characterized the cytochrome P450 genes transcribed in the midgut of the caterpillar. A total of 319 million paired-end reads were sequenced from larval midgut tissue, and these were assembled de novo into 186,000 contigs. Identification of putative P450s in this assembly was carried out using BLAST homology. As detoxification of phytochemicals, mycotoxins, and pesticides is mediated at least in part by P540 enzymes, developing genomic resources for characterizing P450s and elucidating their function in this insect will aid in management efforts for this pest. An important component of elucidating P450-mediated detoxification in this highly polyphagous species is to understand how ingestion of multiple types of toxins influences detoxification. One pyrethroid pesticide used in almond orchards, α-cypermethrin, is likely detoxified by P450 genes transcribed in the navel orangeworm midgut. Dietary exposure to two phytochemicals found in almonds and pistachios, chlorogenic acid and quercetin, increases survival of navel orangeworms exposed to α-cypermethrin. These phytochemicals may decrease toxicity of the pesticide by inducing the P450s involved in its detoxification. Decreased effectiveness of α-cypermethrin is a concern to almond and pistachio growers. Characterization of the P450s involved in pesticide detoxification in the navel orangeworm on its multiple host plants can inform pesticide management strategies for this pest. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-07-09T21:39:22Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Noble_Katherine.docx: 1598243 bytes, checksum: 68475abf7497157753c4f3d1f09a2479 (MD5) Noble_Katherine.pdf: 1245803 bytes, checksum: a176d1608e6041ca07fe172b5e975085 (MD5) Made available in DSpace on 2013-08-22T16:48:17Z (GMT). No. of bitstreams: 3 Katherine_Noble.pdf: 1245803 bytes, checksum: a176d1608e6041ca07fe172b5e975085 (MD5) Noble_Katherine.docx: 1598243 bytes, checksum: 68475abf7497157753c4f3d1f09a2479 (MD5) license.txt: 4064 bytes, checksum: 3a467bcf683f67d306a533e09fb7553a (MD5) Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Seth Robbins (srobbins@illinois.edu) on 2013-08-22T16:49:38Z Item is restricted until 2015-08-22T16:49:27Z Restriction data tranferred 2014-07-01T11:21:12-05:00 Original Data Group with Access UIUC Users [automated] Release Date: 2015-08-22 11:49:27 UTC Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system U of I Only Restriction Lifted for Item 45556 on 2015-08-22T10:00:50Z. http://hdl.handle.net/2142/45574 en Copyright 2013 Katherine Noble Cytochrome P450 Detoxification Lepidoptera: Amyelois transitella: Midgut Transcriptome Xenobiotic detoxification in the navel orangeworm Amyelois transitella (Lepidoptera: Pyralidae) text Entomology 1361 Entomology 0324 University of Illinois at Urbana-Champaign Thesis M.S. MS:Entomology -UIUC 10KS0324MS