2026-09-21T10:01:32Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/45631 2023-07-11 col_2142_5131 col_2142_14770 com_2142_5130 com_2142_3518 com_2142_234

Liu, Wen-Tso Nobu, Masaru 2013-08-22T16:56:02Z 2013-08-22T16:56:02Z 2015-08-22T10:00:26Z 2013-08 2013-08-22T16:56:02Z 2013-08 In an acetate-fed anaerobic-aerobic membrane bioreactor with deteriorated enhanced biological phosphorus removal (EBPR), Defluviicoccus-related tetrad-forming organisms (DTFO) were observed to predominate in the microbial community. Using metagenomics, a partial genome of the predominant DTFO, “Candidatus Defluviicoccus tetraformis TFO71,” was successfully constructed and characterized. Examining the genome confirmed the presence of genes related to the synthesis and degradation of glycogen and polyhydroxyalkanoate (PHA), which function as energy and carbon storage compounds. Both TFO71 and Candidatus Accumulibacter phosphatis (CAP) UW-1, a representative polyphosphate-accumulating organism (PAO), have PHA metabolism-related genes with high homology, but TFO71 has unique genes for PHA synthesis, gene regulation, and granule management. We further discovered genes encoding DTFO polyphosphate (polyP) synthesis, suggesting that TFO71 may synthesize polyP under untested conditions. However, TFO71 may not activate these genes under EBPR conditions because the retrieved genome does not contain inorganic phosphate transporters that are characteristic of PAOs (CAP UW-1, Microlunatus phosphovorus NM-1, and Tetrasphaera species). As a first step in characterizing EBPR-associated DTFO metabolism, this study identifies important differences between TFO and PAO that may contribute to EBPR community competition and deterioration. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-06-28T14:37:31Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Nobu_Masaru.pdf: 7767411 bytes, checksum: 4365353096aae70cbaaedda61560e9f2 (MD5) Made available in DSpace on 2013-08-22T16:56:02Z (GMT). No. of bitstreams: 2 Masaru_Nobu.pdf: 7753753 bytes, checksum: 61689ea38fd190d35018800e78ff7647 (MD5) license.txt: 4058 bytes, checksum: d80fd9322ee87fcc4060766e67955737 (MD5) Restriction data tranferred 2014-07-01T11:36:26-05:00 Original Data Group with Access Administrator Release Date: 2015-08-22 11:57:26 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Item marked as restricted to the 'Administrator' Group (id=1) by Seth Robbins (srobbins@illinois.edu) on 2013-08-22T16:57:33Z Item is restricted until 2015-08-22T16:57:26Z Limited Restriction Lifted for Item 45613 on 2015-08-22T10:00:26Z. http://hdl.handle.net/2142/45631 en Copyright 2013 Masaru Nobu Enhanced biological phosphorus removal Defluviicoccus tetrad-forming organisms glycogen-accumulating organisms Metagenomic characterization of Candidatatus Defluviicoccus tetraformis TFO71, a tetrad-forming organism, predominant in an anaerobic-aerobic membrane bioreactor with deteriorated biological phosphorus removal text Civil & Environmental Eng 1251 Environ Engr in Civil Engr 0231 University of Illinois at Urbana-Champaign Thesis M.S. MS:Env Engr Civil Engr -UIUC 10KS0231MS