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        <identifier>oai:www.ideals.illinois.edu:2142/46652</identifier>
        <datestamp>2023-07-11</datestamp>
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          <dc:contributor>Grift, Tony E.</dc:contributor>
          <dc:creator>Reis, Robert</dc:creator>
          <dc:date>2014-01-16T17:57:36Z</dc:date>
          <dc:date>2014-01-16T17:57:36Z</dc:date>
          <dc:date>2013-12</dc:date>
          <dc:date>2014-01-16T17:57:36Z</dc:date>
          <dc:date>2013-12</dc:date>
          <dc:description>The quantitative measurement of plant features, also known as “phenotyping”, is an
important step in the development of improved varieties of corn. These improvements typical
consist of increased yield, but also increased resistance to biotic and abiotic stresses is paramount.
The speed of this crop improvement is currently limited by rather archaic methods of
phenotyping, often consisting of manual measurements of morphological features. In this
research, a new addition to the technology of phenotyping was developed. Earlier, image based
high-throughput phenotyping methods have been developed that allow for the measurement of
root complexity, stalk diameter and root angle. The method used is direct imaging of the root
structures under controlled diffuse lighting. The method as developed in this work, differs in that
the lighting is not diffuse, but structured in the form of a laser sheet that is projected through a
root system, where the illuminated areas are recorded using a digital camera. By moving the
laser sheet to vertically different areas of the root structure were illuminated. The root was also
rotated, allowing for several sides of the root to be scanned and the resulting angle to be based on
multiple views of the root. The same procedure was applied to tassels. The imagery was
analyzed to obtain root angles that were compared to root angles measured using the direct
imaging method.
Important issues for future development were identified, including sample preparation
procedures, the requirements of all systems, and improvements to the technology.</dc:description>
          <dc:description>Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2013-12-13T17:17:17Z
Item was in collections:
University of Illinois Theses &amp; Dissertations (ID: 1)
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          <dc:identifier>http://hdl.handle.net/2142/46652</dc:identifier>
          <dc:language>en</dc:language>
          <dc:rights>Copyright 2013 Robert Reis</dc:rights>
          <dc:subject>Maize</dc:subject>
          <dc:subject>Corn Root</dc:subject>
          <dc:subject>Phenotyping</dc:subject>
          <dc:title>Evaluation of phenotyping methods for maize</dc:title>
          <dc:type>text</dc:type>
          <degree>
            <department>Engineering Administration</department>
            <departmentCode>1227</departmentCode>
            <discipline>Agricultural &amp; Biological Engr</discipline>
            <disciplineCode>5163</disciplineCode>
            <grantor>University of Illinois at Urbana-Champaign</grantor>
            <level>Thesis</level>
            <name>M.S.</name>
            <program>MS: Agr &amp; Biol Engr -UIUC</program>
            <programCode>10KS5163MS</programCode>
          </degree>
        </thesis>
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