2026-09-19T12:24:17Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/46824 2023-07-11 col_2142_5131 col_2142_16308 com_2142_5130 com_2142_16307 com_2142_8913 com_2142_8903

Pittendrigh, Barry R. Vossbrinck, Alice 2014-01-16T18:17:23Z 2014-01-16T18:17:23Z 2016-01-16T11:02:27Z 2013-12 2014-01-16T18:17:23Z 2013-12 The cowpea bruchid, Callosobruchus maculatus is a major pest of the cowpea, which is an important crop in many areas of Africa and is also consumed in other parts of the world. The cowpea utilizes serine peptidase inhibitors as a defense against herbivorous insects. Peptidases have been considered a target for pest control. The cowpea bruchid in turn employs cysteine peptidases as its major digestive peptidase to avoid the plant’s defense. Important not only in digestion, but in other functions such as immune response and molting, the peptidase and non-peptidase homologs were annotated. Here a de novo transcriptome assembly was built from 840,766 Roche 454 reads using CLC Genomics workbench. The assembly resulted in 35,111 contigs that were analyzed using Blast2GO. Important not only in digestion, but in other functions such as immune response and molting, the peptidase and non-peptidase homologs were annotated. A local BLAST search against sequences downloaded from the MEROPS database found 264 putative peptidase and non-peptidase homologs for C. maculatus. Illumina sequencing from the same sample produced 295,690,882 reads which were mapped back to the assembly using BWA software. While C. maculatus does not appear to have an increased number of cysteine peptidases compared with other insects it does appear have more highly expressed cysteine peptidases compared to serine peptidases. Examination of the peptidase and non-peptidase homolog composition of C. maculatus might ultimately lead to innovations in pest management or other biological insights. Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2013-12-13T16:54:54Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Vossbrinck_Alice.docx: 628536 bytes, checksum: 6b5be93aeba2038ae014c9fec61ccba6 (MD5) Vossbrinck_Alice.pdf: 1041986 bytes, checksum: 3173f39cba41f16eb75349309307d16f (MD5) Made available in DSpace on 2014-01-16T18:17:23Z (GMT). No. of bitstreams: 3 Alice_Vossbrinck.pdf: 1336862 bytes, checksum: 9f96a1d9eb45986fb2db9e570c002960 (MD5) Vossbrinck_Alice.docx: 649875 bytes, checksum: aa51995fa0895f124653844c7c35d772 (MD5) license.txt: 4066 bytes, checksum: 06a81ab433d2de72d9fb2a4799f79a3e (MD5) Restriction data tranferred 2014-07-01T11:36:09-05:00 Original Data Group with Access UIUC Users [automated] Release Date: 2016-01-16 12:19:34 UTC Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Seth Robbins (robbins.sd@gmail.com) on 2014-01-16T18:19:42Z Item is restricted until 2016-01-16T18:19:34Z U of I Only Restriction Lifted for Item 46843 on 2016-01-16T11:02:27Z. http://hdl.handle.net/2142/46824 en Copyright 2013 Alice Vossbrinck transcriptome cowpea brucid Callosobruchus maculatus protease peptidase non-peptidase homolog Characterization of the transcriptome and the peptidase and the non-peptidase homologs of callosobruchus maculatus text Entomology 1361 Entomology 0324 University of Illinois at Urbana-Champaign Thesis M.S. MS:Entomology -UIUC 10KS0324MS