2026-09-24T02:03:26Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/46841 2023-07-11 col_2142_5131 col_2142_14737 com_2142_5130 com_2142_690 com_2142_397

Hartman, Glen L. Zhao, Youfu Hartman, Glen L. Domier, Leslie L. Kent, Angela D. Yang, Hui-Ching 2014-01-16T18:18:06Z 2014-01-16T18:18:06Z 2016-01-16T11:01:04Z 2013-12 2014-01-16T18:18:06Z 2013-12 Colletotrichum species are filamentous fungi. Many species are important pathogens causing economic losses on a wide range of crop plants. Diseases caused by Colletotrichum species often are referred to as anthracnose. The species associated with soybean [Glycine max (L.) Merr.] have not been sufficiently studied in terms of their genotype discrimination and phylogenetic relationships. Moreover, there is little information on evaluation of soybean germplasm accessions for anthracnose resistance. Colletotrichum species were isolated from soybean petioles and stems with anthracnose symptoms from soybean fields in several states in the United States. Multi-gene sequence phylogenetic analyses resulted in a distinct group of isolates that were separated from other known Colletotrichum species. A representative isolate from this group was examined for its morphology, cultural characteristics, and pathogenicity on soybean. The phylogenetic and morphological analyses showed that this group was a new species and was named Colletotrichum incanum. Real-time PCR assays based on dsDNA-binding dye were developed using a region of the cytochrome c oxidase subunit 1 (cox1) gene to discern four Colletotrichum species, C. chlorophyti, C. incanum, C. truncatum, and Glomerella glycines (Colletotrichum sp.). To increase detection efficiency, two sets of duplex real-time PCR assays were established. Set 1 duplex assay distinguished C. chlorophyti and G. glycines, and the Set 2 duplex assay distinguished C. incanum and C. truncatum. Successful detection was achieved with a minimum of 1 pg DNA. The duplex assays rapidly and effectively identified more than 200 Colletotrichum isolates collected from soybean. An inoculation technique for evaluating soybeans for anthracnose resistance was optimized based on experiments to determine the type of inoculum (conidia vs. mycelia), duration of wetness of inoculated plants, and the age of inoculated plants. This technique was used to reevaluate previously reported anthracnose resistant soybean genotypes and a set of ancestral genotypes of modern day soybean cultivars. In the re-evaluation test of previously assessed soybean genotypes, Mandarin, Mandarin 507, and Mandarin (Ottawa) showed greater resistance than the other genotypes. Among the 14 ancestral lines tested, Mandarin had lowest disease rating, although it did not differ from Mandarin (Ottawa), CNS, Roanoke, Richland, and Mukden. Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2013-10-28T22:05:53Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Yang_Hui-Ching.docx: 170244987 bytes, checksum: b4f105ae5eac58c77b9dc29aea77fd7f (MD5) Yang_Hui-Ching.pdf: 36094336 bytes, checksum: 7b91c34b608f1a64e97d223bab284dea (MD5) Made available in DSpace on 2014-01-16T18:18:06Z (GMT). No. of bitstreams: 3 Hui-Ching_Yang.pdf: 36546313 bytes, checksum: 6c58f8e6a921d48382ef171f8912708e (MD5) Yang_HuiChing.docx: 170229891 bytes, checksum: 88dced6a60d2248b2b81b0f154b8520d (MD5) license.txt: 4063 bytes, checksum: e44c960dbde709719a7fcfe131a2f615 (MD5) Restriction data tranferred 2014-07-01T11:36:36-05:00 Original Data Group with Access UIUC Users [automated] Release Date: 2016-01-16 12:19:34 UTC Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Seth Robbins (robbins.sd@gmail.com) on 2014-01-16T18:19:47Z Item is restricted until 2016-01-16T18:19:34Z U of I Only Restriction Lifted for Item 46860 on 2016-01-16T11:01:04Z. http://hdl.handle.net/2142/46841 en Copyright 2013 Hui-Ching Yang soybean anthracnose Colletotrichum species real-time polymerase chain reaction (PCR) soybean genotypes for anthracnose resistance Morphological and molecular identification, pathogenicity characterization of colletotrichum species on soybean, and the resistance of soybean genotypes text Natural Res & Env Sci 1875 Natural Res & Env Sciences 0190 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Nat Res & Envrn Sci -UIUC 10KS0190PHD