2026-09-21T10:04:05Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/46916 2023-07-11 col_2142_14789 col_2142_5131 com_2142_14788 com_2142_8903 com_2142_5130

Lu, Yi Brown, Darius 2014-01-16T18:26:15Z 2014-01-16T18:26:15Z 2016-01-16T11:02:07Z 2013-12 2014-01-16T18:26:15Z 2013-12 This is a comprehensive project for the development of a general method to enhance the sensitivity of DNA aptasensors by coupling them with an isothermal DNA amplification technique known as exponential amplification reaction (EXPAR). The method involves two steps: (1) first, the target-induced structure-switching of DNA aptamers causes the release of trigger oligonucleotides from the aptamers immobilized on a surface; (2) then, the released trigger oligonucleotides are amplified by the EXPAR technique for ultrasensitive detection. It is shown that the detection limit of a cocaine aptasensor is improved from 5 micromolar in assays without amplification to 0.8-1micromolar when EXPAR is applied, providing a 5-fold enhancement. The flexibility in designing the sequences of the trigger oligonucleotides enables the method generally applicable to other aptasensor systems such as thrombin aptamers. By EXPAR, the method has also achieved 10-fold enhancement in sensitivity from 4nM to 400 pM for thrombin detection, in comparison with similar assays without EXPAR. Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2013-12-02T16:11:37Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Brown_DariusFINAL of Finals.doc: 3492352 bytes, checksum: 4f43387ae06fbcba88ede6a5c5c7e81d (MD5) Brown_Darius.pdf: 1183195 bytes, checksum: 99e25f4b0f8067357205e9c845bd914e (MD5) Made available in DSpace on 2014-01-16T18:26:15Z (GMT). No. of bitstreams: 3 Darius_Brown.pdf: 1182350 bytes, checksum: a26aa96ae39fdd593c9d19d217e877bf (MD5) Brown_DariusFINAL of Finals.doc: 3492352 bytes, checksum: 4f43387ae06fbcba88ede6a5c5c7e81d (MD5) license.txt: 4062 bytes, checksum: 85c5f44a6d807620671099fd97de763a (MD5) Item marked as restricted to the 'Administrator' Group (id=1) by Seth Robbins (robbins.sd@gmail.com) on 2014-01-16T18:27:36Z Item is restricted until 2016-01-16T18:27:27Z Restriction data tranferred 2014-07-01T11:36:49-05:00 Original Data Group with Access Administrator Release Date: 2016-01-16 12:27:27 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 46935 on 2016-01-16T11:02:07Z. http://hdl.handle.net/2142/46916 en Copyright 2013 Darius Z Brown Aptasensor Cocaine Thrombin Exponential amplification reaction (EXPAR) Magnetic Beads Deoxyribonucleic acid (DNA) Ultrasensitive DNA aptasensors based on exponential amplification reaction text Chemistry 1413 Chemistry 0335 University of Illinois at Urbana-Champaign Thesis M.S. MS:Chemistry -UIUC 10KS0335MS