2026-09-22T08:25:29Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/46921 2023-07-11 col_2142_14789 col_2142_5131 com_2142_14788 com_2142_8903 com_2142_5130

Sligar, Stephen G. Baker, Catherine 2014-01-16T18:26:24Z 2014-01-16T18:26:24Z 2016-01-16T11:02:18Z 2013-12 2014-01-16T18:26:24Z 2013-12 Nanodiscs, engineered lipoparticles composed of a lipid bilayer and two surrounding amphipathic proteins, have been utilized to house and solubilize integral membrane proteins and other molecules with large hydrophobic groups which limit their aqueous solubility. In the experiments described herein, lipids from the Nanodiscs’ bilayer, gadolinium chelates and integral membrane proteins were analyzed for their incorporation and stability within the Nanodisc structure. Four techniques are described for quantification of the exchange of lipids between Nanodiscs over time, including: scintillation counting, imaging proximity assay, NMR and TLC MALDI-MS. Additionally, the extent and longevity of inclusion of the hydrophobic gadolinium-chelate “C3lipo” doped into the Nanodisc bilayer was analyzed and preliminary cell uptake studies were performed. Finally, the incorporation of proteins from rat liver and fetal bovine brain membranes into Nanodiscs to create soluble membrane protein libraries is discussed. Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2013-12-02T18:56:27Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Baker_Catherine.docx: 12584468 bytes, checksum: 111d926351f61a4b63beb88def9ca588 (MD5) Baker_Catherine.pdf: 1750657 bytes, checksum: 956bc11650444c8f602d2bb9361968ec (MD5) Made available in DSpace on 2014-01-16T18:26:24Z (GMT). No. of bitstreams: 4 Catherine_Baker.pdf: 1926245 bytes, checksum: 4033f04a4dc1fef02dd9d8a9a16de8c3 (MD5) Baker_Catherine.docx: 12585471 bytes, checksum: c7ed14ce9aa0dfb2c9a4fe33ee48d55e (MD5) 1_Baker_Catherine.docx: 12584468 bytes, checksum: 111d926351f61a4b63beb88def9ca588 (MD5) license.txt: 4065 bytes, checksum: 30421e5c1b513063bc4c61853108c250 (MD5) Item marked as restricted to the 'Administrator' Group (id=1) by Seth Robbins (robbins.sd@gmail.com) on 2014-01-16T18:27:38Z Item is restricted until 2016-01-16T18:27:27Z Restriction data tranferred 2014-07-01T11:36:50-05:00 Original Data Group with Access Administrator Release Date: 2016-01-16 12:27:27 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 46940 on 2016-01-16T11:02:18Z. http://hdl.handle.net/2142/46921 en Copyright 2013 Catherine Baker Nanodiscs Lipid exchange Soluble membrane protein library (SMPL) Lipoprotein Membrane scaffold protein Gadolinium Incorporation and analysis of bilayer constituents in nanodiscs text Chemistry 1413 Chemistry 0335 University of Illinois at Urbana-Champaign Thesis M.S. MS:Chemistry -UIUC 10KS0335MS