2026-09-20T11:42:46Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/49844 2023-07-11 col_2142_5163 col_2142_5131 com_2142_5162 com_2142_234 com_2142_5130

Cunningham, Brian T. Long, Kenneth 2014-05-30T17:20:41Z 2014-05-30T17:20:41Z 2016-09-22T20:59:31Z 2014-05 2014-05-30T17:20:41Z 2014-05 This thesis serves to introduce a new point-of-care testing device capable of providing spectrometric data for solid and liquid phase samples. A custom cradle was developed to maintain optical alignment allowing for the diffraction, and therefore spectrometric analysis, of light transmitted either directly through a sample or via an intermediary optical biosensor. Demonstration of the instrument is provided with both an enzyme-linked immunosorbent assay (ELISA) and a photonic-crystal (PC)-based investigation of biomolecular adsorption. ELISA analysis is completed on both human interleukin-6, an important diagnostic cancer biomarker, and Ara h 1, one of the principle peanut proteins responsible for allergic reactions. PC-based sensing was completed with a Protein A-immunoglobulin G interaction with a .009 nm accuracy. All of these demonstrations were completed at physiologically-relevant concentrations, and as such, serve as proof-of-concept for two separate modalities of a smartphone-based spectrometric optical biosensor. In the future, such a sensor has the potential to provide a low-cost, point-of-care diagnostic device that is inherently robust, user-friendly, cloud-connected, and suited for use in remote sensing applications. Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-04-29T13:13:30Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Long_Kenneth.docx: 13640274 bytes, checksum: 37040d61a668b7fae04703d10bd1a0d3 (MD5) Long_Kenneth.pdf: 1249364 bytes, checksum: d16fa43176ad783648a663022430aa89 (MD5) Made available in DSpace on 2014-05-30T17:20:41Z (GMT). No. of bitstreams: 3 Kenneth_Long.pdf: 1252755 bytes, checksum: d8cf960c11296bec78aa5e93120e80f4 (MD5) Long_Kenneth.docx: 13639440 bytes, checksum: a4e3f4cf102f6730f6a2d639cd668738 (MD5) license.txt: 4059 bytes, checksum: 04bd5a61c6d6e9fa462bd6e9293cc27f (MD5) Item marked as restricted to the 'Administrator' Group (id=1) by Seth Robbins (robbins.sd@gmail.com) on 2014-05-30T17:21:46Z Item is restricted until 2016-05-30T17:21:23Z Restriction data tranferred 2014-07-01T11:39:50-05:00 Original Data Group with Access Administrator Release Date: 2016-05-30 12:21:23 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 49895 on 2016-09-22T20:59:31Z. http://hdl.handle.net/2142/49844 en Copyright 2014 Kenneth D. Long Smartphone Biosensing Photonic Crystal Enzyme-Linked Immunosorbent Assay Mobile Biosensing Point-of-Care Diagnostics A spectroscopic smartphone biosensor for use in point-of-care diagnostic applications text Bioengineering 1343 Bioengineering 0408 University of Illinois at Urbana-Champaign Thesis M.S. MS:Bioengineering - UIUC 10KS0408MS