2026-09-23T16:56:44Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/50374 2023-07-11 col_2142_5131 col_2142_14781 com_2142_5130 com_2142_687 com_2142_397

Miller, Michael J. Berquist, Allison 2014-09-16T17:12:17Z 2014-09-16T17:12:17Z 2016-09-22T20:59:16Z 2014-08 2014-09-16 2014-08 Campylobacter jejuni is one of the leading causes of foodborne bacterial illness in the world. The majority of cases of campylobacteriosis are associated with the consumption of raw or undercooked poultry meat or their cross-contamination with other food products. Strategies to reduce the presence of Campylobacter in the poultry supply are underdeveloped. The probiotic Lactobacillus spp. are a viable delivery vehicle for mucosal vaccines due to the Generally Recognized As Safe (GRAS) status. In addition, recombinant lactobacilli present proteins to the mucosal surface and elicit host immune responses. The overall objectives of this research were to 1) colonize broiler chickens with C. jejuni; 2) construct recombinant Lactobacillus for oral delivery of vaccines against C. jejuni; and 3) evaluate the immunogenicity of the recombinant Lactobacillus strains in broiler chickens. To meet these objectives, broiler chickens were orally administered with 109 CFU/mL of C. jejuni NCTC 11168 on d 14, then necropsied on d 21 for direct-plating of the cecal contents. Results indicate successful C. jejuni colonization of the cecal crypts. Lactobacillus strains were engineered to express C. jejuni FlpA Domain II under a promoter fused to a secretion signal with or without an anchoring signal from the mucus-binding protein of Lactobacillus acidophilus NCFM. Additional work must be conducted to evaluate the exterior protein expression and in vivo antigen-immune response within broiler chickens. If successful, the recombinant Lactobacillus strain could be an attractive candidate as a food-grade live vaccine against Campylobacter colonization. In addition, a non-genetically modified delivery approach proposes attachment of C. jejuni FlpA to the cell wall of lactobacilli utilizing one of three cell wall binding domains. Reduction of Campylobacter at the poultry farm decreases the introduction of the bacteria into the retail market and decreases the incidence of campylobacteriosis. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2014-07-11T19:03:39Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Berquist_Allison.docx: 1944164 bytes, checksum: bd9ecee3f22926d1596f48029aa2d363 (MD5) Berquist_Allison.pdf: 2337840 bytes, checksum: 835f3d96ef110a00c3ed1544536b714a (MD5) Made available in DSpace on 2014-09-16T17:12:17Z (GMT). No. of bitstreams: 3 Allison_Berquist.pdf: 2337840 bytes, checksum: 835f3d96ef110a00c3ed1544536b714a (MD5) Berquist_Allison.docx: 1944164 bytes, checksum: bd9ecee3f22926d1596f48029aa2d363 (MD5) license.txt: 4066 bytes, checksum: 649bc9865f624f92e4334798a3e49348 (MD5) Embargo set by: Seth Robbins for item 50485 Lift date: 2016-09-16T17:13:01Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 50485 on 2016-09-22T20:59:16Z. http://hdl.handle.net/2142/50374 en Copyright 2014 Allison Berquist Campylobacter Lactobacillus probiotic vaccine chicken Lactobacillus strategies to reduce Campylobacter jejuni colonization of broiler chickens text Food Science & Human Nutrition 1698 Food Science & Human Nutrition 0037 University of Illinois at Urbana-Champaign Thesis M.S. MS:FSci &Hmn Nutrition -UIUC 10KS0037MS