2026-09-25T08:26:50Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/69995 2023-07-11 col_2142_5131 col_2142_14771 com_2142_5130 com_2142_685 com_2142_397

Noble, Ralph C. 2014-12-15T21:00:40Z 2014-12-15T21:00:40Z 10000-01-01 1982 1982 A pregnancy specific antigen was isolated from feto-placental units and sera of early pregnant cows. Antibodies produced against this antigen were used in tests for determination of pregnancy on days 7-277. The presence of the antigen in milk and urine of early pregnant cows was also tested. Sera from heifers and steers were evaluated to determine the specificity of the antigen. Results, using antibodies generated against the feto-placental unit, indicate that a pregnancy specific antigen is present in sera of pregnant cows on days 14, 21, and 30. The qualitative precipitatin test was 77% accurate in identifying pregnant cows. A pregnancy specific antigen was isolated from day 14 cow sera by affinity chromatography with Protein A-Sepharose CL-4B and identified by crossed immunoelectrophoresis. Antibodies against this antigen were used in the capillary precipitatin test to detect pregnancy on days 14-277. No precipitate was observed in milk or urine from early pregnant cows. The greatest accuracy was obtained when results were read 1 hour after mixing antibodies with sera. Results from reacting sera from heifers and steers with antibodies yielded a slight precipitate that was similar to non-pregnant cows. Results show that a pregnancy specific antigen is circulating in the sera of pregnant cows on day 14 of pregnancy. Antibodies against this antigen can be employed in a pregnancy test that is superior to chance in identifying pregnancy as early as day 14 and throughout gestation, but not in identifying nonpregnant cows. Pregnancy could not be confirmed by testing milk or urine. Made available in DSpace on 2014-12-15T21:00:40Z (GMT). No. of bitstreams: 1 8218531.pdf: 2963551 bytes, checksum: 6530e8d8248a621f18e4debfbc2be743 (MD5) Previous issue date: 1982 Embargo set by: Seth Robbins for item 70161 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 94 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982. http://hdl.handle.net/2142/69995 (UMI)AAI8218531 Agriculture, Animal Culture and Nutrition The Isolation and Utilization of a Pregnancy Specific Antigen for Early Pregnancy Detection in The Bovine Species text Animal Sciences Animal Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.