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Pilosof, David 2014-12-15T23:17:49Z 2014-12-15T23:17:49Z 10000-01-01 1982 1982 Microporous membrane chemiluminescence (CL) flow cells are presented as an advantageous method for chemical reagent and analyte mixing in continuous flow. The method adds simplicity, significant reagent economy and minimal sample contamination to the conventional advantages of analytical CL determinations. The most significant application of the proposed method is in coupling enzymatic reactions that generate hydrogen peroxide to CL detection, in the same flow cell, in spite of the very different pH optima required by both processes. The analysis of glucose, using this approach, is studied in depth, and results obtained are compared to values determined by a routine assay method with good correlation. Other applications include analysis by use of other oxidase enzymes, determination of mixtures and determination of sugars that can be enzymatically converted to D-glucose. Made available in DSpace on 2014-12-15T23:17:49Z (GMT). No. of bitstreams: 1 8302961.pdf: 4811854 bytes, checksum: 3c5dac22ac75f73d3391a0eaf88dfa26 (MD5) Previous issue date: 1982 Embargo set by: Seth Robbins for item 70370 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 175 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982. http://hdl.handle.net/2142/70204 (UMI)AAI8302961 Chemistry, Analytical Analytical Chemiluminescence in Microporous Membrane Flow Cells text Chemistry Chemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.