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oai:www.ideals.illinois.edu:2142/70565 2023-07-11 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

Cioffi, Linda Carol 2014-12-15T23:44:05Z 2014-12-15T23:44:05Z 10000-01-01 1987 1987 Chondrocytes synthesize and secrete a cartilage-specific proteoglycan (PG-H) as one of their major products. This proteoglycan has attached to it several types of carbohydrate chains, including chondroitin sulfate, keratan sulfate, O-linked oligosaccharides, and asparagine-linked oligosaccharides. The asparagine-linked oligosaccharides found on PG-H were investigated in these studies. Methodology was developed for the isolation and separation of standard of standard complex and high mannose type oligosaccharides. This included digesting glycoproteins with N-glycanase and separation of the oligosaccharides according to type by concanavalin-A lectin chromatography. The different oligosaccharide types were then analyzed by high pressure liquid chromatography. This methodology was used in the subsequent studies on the PG-H asparagine-linked oligosaccharides. Initially, the asparagine-linked oligosaccharides recovered from the culture medium (CM) and cell-associated (Ma) fractions of PG-H from of tibial chondrocytes were labeled with ($\sp3{\rm H}$) -mannose and the oligosaccharides were isolated and analyzed. The asparagine-linked oligosaccharides were found to be heterogeneous mixtures of complex and high mannose structures in both the CM and Ma PG-H pools. The ratio of complex to high mannose type oligosaccharides was found to be 6.1 in the CM PG-H and 2.6 in the Ma PG-H. These results suggest the possibility that the CM and Ma PG-H are two separate and distinct molecules, each having its own structure and function. The chick embryo tibiotarsus was then dissected into zones which differed in their states of differentiation. The PG-H from monolayer cultures of these distinct zones of chondrocytes were isolated and the asparagine-linked oligosaccharides from the CM and Ma pools were characterized are found to be heterogeneous mixtures of oligosaccharide structures. The PG-H from each zone gave its own characteristic array of structures. In addition to cell culture studies, the asparagine-linked oligosaccharide structures from PG-H in tissue culture were investigated. In every system analyzed--mixed zone cell culture, distinct zone cell culture, mixed zone embryo tibial tissue, and the distinct zones of the chick embryo tibial tissue--the asparagine-linked oligosaccharides were found to be a heterogeneous mixture of complex and high mannose structures. Made available in DSpace on 2014-12-15T23:44:05Z (GMT). No. of bitstreams: 1 8721610.pdf: 5135947 bytes, checksum: 647390e674d4f90293d9d4e5dccb7e06 (MD5) Previous issue date: 1987 Embargo set by: Seth Robbins for item 70731 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 192 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987. http://hdl.handle.net/2142/70565 (UMI)AAI8721610 Chemistry, Biochemistry Studies on the Asparagine-Linked Oligosaccharides From Cartilage-Specific Proteoglycan (Chondrocytes) text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.