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115 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987. http://hdl.handle.net/2142/71188 (UMI)AAI8803241 Biology, Molecular Scott, John F., Woontner, Michael Roger 2014-12-16T06:13:01Z 2014-12-16T06:13:01Z 10000-01-01 1987 1987 Autonomously Replicating Sequences, or ARS elements, promote high-frequency transformation and extrachromosomal maintenance of plasmids in Saccharomyces cerevisiae, properties expected of DNA replication origins. A series of overlapping deletions in one flanking region (Domain C) of the ARSl element was constructed, and the effect of the deletions on the maintenance of various multicopy and single-copy plasmids examined. Analysis of the stabilities and copy numbers of multicopy plasmids indicated that while the core consensus element is absolutely required for extrachromosomal maintenance, the absence of Domain C has little effect. The loss rates of centromere (single-copy) plasmids increased slightly as the deletions approached the core consensus, suggesting that a block of sequence between 225 and 255 nucleotides from the consensus contains an element important to the maximal function of ARSl. These results also suggested that ARSl plays a role in replication, but has no effect on the segregation of centromere plasmids. Comparison of multicopy plasmids of different sizes, each with an intact ARSl element, indicated a destabilizing effect of sequences derived from the E. coli cloning vector pBR322. A small amount of pBR322 (about a kilobase) was tolerated, and the bacterial ori was not responsible for the destabilizing effect. Made available in DSpace on 2014-12-16T06:13:01Z (GMT). No. of bitstreams: 1 8803241.pdf: 3849495 bytes, checksum: 9f936a56720c80f33e27202bc97a7ca2 (MD5) Previous issue date: 1987 Embargo set by: Seth Robbins for item 71354 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only Construction and Characterization of Deletion Mutations in Domain C of ARSl text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.