2026-09-20T15:46:25Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/71441 2023-07-11 col_2142_5131 col_2142_16508 com_2142_5130 com_2142_16507 com_2142_14793 com_2142_8903

text Mcbride, Robert Kenneth 2014-12-16T06:45:50Z 2014-12-16T06:45:50Z 10000-01-01 1986 1986 Experimental animals were injected intraperitoneally with methotrexate (15 mg/kg) for 3 days. Parotid and submandibular main ducts were cannulated and saliva flow was evoked by either intravenous infusion of acetylcholine (10 to 100 (mu)g/min) or an intravenous injection of bethanechol (100 (mu)g/kg). Methotrexate was found to reduce significantly mean food consumption, body weight, and parotid gland wet weights. Experimental animal salivary total gland DNA levels were not different, but total parotid gland RNA, protein, amylase and water content, and submandibular gland RNA were significantly lower compared to controls. Acetylcholine, but not bethanechol, evoked parotid protein and amylase outputs and submandibular protein output from experimental animals were significantly higher than the control groups'. The increased outputs were apparently linked to (beta)-adrenergic receptor activation, since hexamethonium or propranolol eliminated the significant increases while phenoxybenzamine did not. Plasma catecholamine levels were significantly higher in the methotrexate treated animals and probably played a role in the salivary gland (beta)-adrenergic activation. Methotrexate treatment significantly increased the submandibular gland (beta)-adrenergic receptor concentration as determined by ('3)H -dihydroalprenolol receptor binding assays. Muscarinic receptor concentrations determined with ('3)H -quinuclidinyl benzilate were not changed. Made available in DSpace on 2014-12-16T06:45:50Z (GMT). No. of bitstreams: 1 8610961.pdf: 2553694 bytes, checksum: 11e8ce2eeab895ca675413ef6200d346 (MD5) Previous issue date: 1986 Embargo set by: Seth Robbins for item 71607 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 104 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1986. http://hdl.handle.net/2142/71441 (UMI)AAI8610961 Health Sciences, Pharmacology The Effects of Methotrexate on Rat Parotid and Submandibular Glands and Their Secretions (Antineoplastic, Protein, Salivary, Amylase Catecholamines) Physiology and Biophysics Physiology University of Illinois at Urbana-Champaign Dissertation Ph.D.