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oai:www.ideals.illinois.edu:2142/71443 2023-07-11 col_2142_5131 col_2142_16508 com_2142_5130 com_2142_16507 com_2142_14793 com_2142_8903

Sandborg, Rebecca Ruth 2014-12-16T06:45:50Z 2014-12-16T06:45:50Z 10000-01-01 1986 1986 Experimental animals were injected intraperitoneally with 100 mg/kg 5-fluorouracil for three days. The total volume, amylase and protein content of cannulated parotid saliva were determined following stimulation with either 5 mg/kg pilocarpine or 5 mg/kg isoproterenol in experimental, pair-fed, and control animals. Saliva from experimental animals was significantly lower in volume, amylase and protein content than both control groups. Sodium dodecyl sulfate, anionic and cationic gel electrophoresis of parotid saliva revealed no qualitative alterations in the types of proteins secreted. 5-fluorouracil treatment reduced the total glandular amylase per unit DNA in both unstimulated and isoproterenol-stimulated parotid glands. Decreased protein synthesis may be the mechanism underlying depleted secretory protein stores since the contents of isolated secretory granules from experimental parotid glands contained less radiolabelled protein than either control group and whole gland homogenates showed marked reductions in the activities of three lysosomal enzymes and total RNA content. Experimental animals contained less labelled protein in their secretory granules than controls, but secreted a greater proportion of their total glandular radiolabelled secretory protein into saliva relative to amylase suggesting that newly synthesized secretory proteins are preferentially secreted. Made available in DSpace on 2014-12-16T06:45:50Z (GMT). No. of bitstreams: 1 8610976.pdf: 3777870 bytes, checksum: 87c02a51c1064a47acafb9899e6b73fc (MD5) Previous issue date: 1986 Embargo set by: Seth Robbins for item 71609 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 133 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1986. http://hdl.handle.net/2142/71443 (UMI)AAI8610976 Biology, Animal Physiology The Effects of 5-Fluorouracil on The Secretory Process of The Rat Parotid Gland (Protein) text Physiology and Biophysics Physiology University of Illinois at Urbana-Champaign Dissertation Ph.D.