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Wagner, William C. Cai, Tian Quan 2014-12-17T23:22:41Z 2014-12-17T23:22:41Z 10000-01-01 1992 1992 Neutrophil functions in periparturient normal cows (n = 46), and their relationship with the occurrence of retained placenta and metritis complex (RPM, n = 20), metritis (n = 18) and mastitis (n = 13), were studied by collecting blood samples via jugular vein twice weekly from 1 week before to 4 weeks after parturition. Seasonal variation of neutrophil function assays (n = 4 ovariectomized cows) and modulation of neutrophil functions in normal and abnormal postpartum cows (RPM, metritis or mastitis, n = 5 per group) were investigated in vitro by using arachidonic acid metabolites ($\rm PGE\sb2:$ 10$\sp{-6}$ M, PGF$\sb{2\alpha}:$ 10$\sp{-7}$ M, LTB$\sb4:$ 10$\sp{-8}$ M, and lipoxin B: 10$\sp{-8}$ M), and recombinant human colony-stimulating factors (rhG-CSF: 1000 and 6000 units/ml, and rhGM-CSF: 5 and 15 ng/ml). The results of these experiments suggest the following conclusions: (1) Random migration and ingestion activity of neutrophils increased, but ADCC and myeloperoxidase activity decreased, which may increase susceptibility to infections; (2) Neutrophils, monocytes and eosinophils decreased after calving; (3) There were seasonal variations in bovine neutrophil functional assays; (4) Neutrophil functions were impaired in abnormal cows after calving. A prepartum defect was noticed in term of superoxide production (metritis) or chemotaxis (mastitis), suggesting the defect of neutrophil function as a predisposing factor in the occurrence of these disorders; (5) Neutrophils decreased after parturition in cows with RPM or metritis, but not with mastitis. Immature neutrophils increased in cows with RPM or mastitis, but not with metritis; (6) In vitro treatment of neutrophils with AA metabolites and rhCSFs did not affect functions of neutrophils from either normal or abnormal postpartum cows; (7) Incubation of neutrophils in a 37$\sp\circ$C water bath for 30 minutes might restore the functions of neutrophils from cows with RPM or metritis, and further impair the functions of neutrophils from cows with mastitis. Made available in DSpace on 2014-12-17T23:22:41Z (GMT). No. of bitstreams: 1 9305478.pdf: 9139756 bytes, checksum: 1acbdcbacd2814297f7946c7ea32a84c (MD5) Previous issue date: 1992 Embargo set by: Seth Robbins for item 72721 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 231 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1992. http://hdl.handle.net/2142/72553 (UMI)AAI9305478 Biology, Animal Physiology Biology, Veterinary Science Bovine Neutrophil Functions and Periparturient Disorders text Veterinary Medical Science Veterinary Medical Science University of Illinois at Urbana-Champaign Dissertation Ph.D.