2026-09-20T20:49:02Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/72771 2023-07-11 col_2142_5131 col_2142_14789 com_2142_5130 com_2142_14788 com_2142_8903

text Zimmerman, Steven C. Zimmerman, Steven C. Hull, Kami L. Mitchell, Douglas A. Murphy, Catherine J. Vincil, Gretchen 2015-01-21T19:48:00Z 2015-01-21T19:48:00Z 2014-12 2015-01-21 2014-12 The photobleaching of fluorophores is a major limitation in fluorescence microscopy. We sought to create a brighter and more stable fluorophore by covalently attaching fluorescein and anti-fading agent (AFA) moieties to hyperbranched polyglycerols (HPG). The use of HPGs as a scaffold to link fluorescein and anti-fading agents proved intractable, so a norbornyl based ROMP polymer was used instead. Through the iterative use of ROMP and RCM, organic nanoparticles (ONPs) were synthesized incorporating multiple fluorescein and AFA units. The ONPs were brighter and more photostable than fluorescein. The photostability of the ONPs without AFAs was found to loosely correlate to its brightness. Incorporation of AFAs in the ONPs led to increased stability in some cases. Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-12-03T16:08:50Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Vincil_Gretchen .pdf: 42839158 bytes, checksum: 8ea85715cf1d25d773720a8bce873892 (MD5) Made available in DSpace on 2015-01-21T19:48:00Z (GMT). No. of bitstreams: 1 Gretchen_Vincil.pdf: 42849042 bytes, checksum: fe1393e7eaf5b6fc84f9ed61abf8ae82 (MD5) http://hdl.handle.net/2142/72771 en Copyright 2014 Gretchen Vincil photobleaching photostability fluorescein anti-fading agents hyper-branched polymers Design, synthesis and characterization of fluorescent hyperbranched polyglycerols and organic nanoparticles with covalently bound anti-fading agents Chemistry 1413 Chemistry 0335 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Chemistry -UIUC 10KS0335PHD