2026-09-21T16:54:09Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/72836 2023-07-11 col_2142_5131 col_2142_14798 com_2142_5130 com_2142_686 com_2142_397

Hudson, Matthew E. Fliege, Christina 2015-01-21T19:48:42Z 2015-01-21T19:48:42Z 2014-12 2015-01-21 2014-12 Glycine soja, the wild annual ancestor of soybean, has great potential to be used as a source of genetic diversity for the crop. A Glycine soja line, PI 468916, contains a resistance QTL to soybean cyst nematode (SCN) Heterodera glycines, a devastating pest of soybean. This resistance QTL has been fine mapped in PI 468916 to a distance of 147kb. Through the use of whole genome sequencing with de novo assembly we were able to gain information about the region. In this thesis the assembly data was used along with fosmid sequences from both Sanger sequencing and Illumina reads to gain insight into sequence differences between the Williams 82 reference genome and PI 468916 to identify the gene(s) responsible for this resistance. The alignment of PI 468916 sequence against the reference genome corresponding to the resistance QTL and the candidate genes present in this region, is presented. This novel resistance locus could be used to introduce new resistance in the crop. Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-12-10T22:38:01Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Fliege_Christina.pdf: 1403134 bytes, checksum: ca88ad879b12cb83b42735c2562a6060 (MD5) Made available in DSpace on 2015-01-21T19:48:42Z (GMT). No. of bitstreams: 1 Christina_Fliege.pdf: 1417365 bytes, checksum: 546e3d1cdbe676b5bb7d98d501310784 (MD5) http://hdl.handle.net/2142/72836 en Copyright 2014 Christina Fliege soybean soybean cyst nematode (SCN) Towards cloning of a novel SCN resistance locus using de novo genome assembly and fosmid screening text Crop Sciences 1802 Crop Sciences 0030 University of Illinois at Urbana-Champaign Thesis M.S. MS:Crop Sciences -UIUC 10KS0030MS