2026-09-21T09:38:01Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/73046 2023-07-11 col_2142_11615 col_2142_5131 com_2142_9130 com_2142_8903 com_2142_5130

poly-mannuronate (polyM) heteropolymeric M/G (polyMG) Rao, Christopher V. Badur, Ahmet 2015-01-21T19:58:51Z 2015-01-21T19:58:51Z 2017-01-22T10:15:38Z 2014-12 2015-01-21 2014-12 lginate is a polysaccharide found within brown seaweeds and has been targeted as a carbon source for biofuel production. Alginate consists of α-L-guluronate (G) and β-D-mannuronate (M) linked in various patterns, which results in either a homo- or heteropolymeric structure. Alginate lyases are enzymes that degrade the linkage between G and M blocks and can have specificity to either polyG, polyM, or polyMG block degradation. The marine bacterium Vibrio splendidus 12B01 contains four putative alginate lyases which were investigated in this study. We identified, purified, and characterized the four PL7 alginates lyases. We found that these lyases have optimal activity between pH 7.5-8.5 and 20-25°C, consistent with use in a marine environment. Additionally, Ca 2+ is necessary for optimal enzyme activity. The binding constant (K m ) of the lyases toward alginate was found to be between 22 and 123 mM alginate and the maximum reaction rate (V max ) was found to be between 0.13 and 0.83 μM s -1 . The turnover numbers for the lyases was found to be between 0.60 and 7.1 s -1 . Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-11-07T22:14:27Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Badur_Ahmet.pdf: 979895 bytes, checksum: acfc73fde53f197e73ef45f2a0d0bc2d (MD5) Made available in DSpace on 2015-01-21T19:58:51Z (GMT). No. of bitstreams: 1 Ahmet_Badur.pdf: 979895 bytes, checksum: acfc73fde53f197e73ef45f2a0d0bc2d (MD5) Embargo set by: Seth Robbins for item 73235 Lift date: 2017-01-21T19:59:39Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 73235 on 2017-01-22T10:15:38Z. http://hdl.handle.net/2142/73046 en Copyright 2014 Ahmet Badur Vibrio splendidus Vibrio splendidus 12B01 alginate alginate lyase poly-guluronate (polyG) polyGM AlyA AlyB AlyC AlyD AlyE Over-expression and characterization of four alginate lyases from vibrio splendidus 12B01 text Chemical & Biomolecular Engr 1687 Chemical Engineering 0300 University of Illinois at Urbana-Champaign Thesis M.S. MS:Chemical Engineering -UIUC 10KS0300MS