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oai:www.ideals.illinois.edu:2142/77444 2023-07-11 col_2142_5131 col_2142_14781 com_2142_5130 com_2142_687 com_2142_397

Basgall, Edward Joseph 2015-05-13T15:44:18Z 2015-05-13T15:44:18Z 10000-01-01 1983 1983 An endothelial cell culture system was used to compare the effects of endogenous arachidonic acid (AA) and eicosapentaenoic acid (EPA) on prostacyclin synthesis. Supplemental levels of fatty acids (20-150 (mu)M) were supplied as sodium salts bound to bovine serum albumin (3:1 mole ratio) and added to M-199 with 20% fetal bovine serum. Both AA and EPA were readily incorporated into neutral and phospholipid fractions to serve as endogenous precursors for prostacyclin (PGI(,2)) synthesis. Thrombin (.5 U/ml) was used to stimulate prostacyclin synthesis. AA enrichment did not result in an increased PGI(,2) synthesis while EPA enrichment decreased PGI(,2) synthesis as measured by radioimmunoassay to 6-keto-PGF(,1(alpha)). Radiolabelled fatty acid eicosanoid products were separated by reverse phase HPLC. Unconverted fatty acid was the major product released upon thrombin stimulation in both cases. ('3)H-AA supplemented cells also released PGI(,2) and HETE in lesser amounts while ('14)C-EPA supplemented cells released only minimal amounts of PGI(,3) and HEPE. The relative percent incorporation of radiolabelled ('3)H-AA and ('14)C-EPA into cellular lipids was determined after two-dimensional thin-layer chromatography. Neutral lipid contained the vast majority of radiolabel ((TURN)50%). Of the phospholipids, phosphatidylcholine contained the greatest percentage of radiolabel. Phosphatidylinositol and phosphatidylethanolamine contained intermediate percentages while phosphatidylserine and sphingomyelin contained the least. In a dual label supplementation regimen (150 (mu)M ('14)C-EPA and 16 (mu)M ('3)H-AA), phosphatidylinositol appeared to be readily saturated with fatty acid at an early stage. This may be an important regulatory aspect for prostaglandin synthesis. Made available in DSpace on 2015-05-13T15:44:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 8409859.PDF: 4495746 bytes, checksum: 418b891588ba19130cfaa1bb803bb968 (MD5) Previous issue date: 1983 Embargo set by: Seth Robbins for item 78655 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 153 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1983. http://hdl.handle.net/2142/77444 (UMI)AAI8409859 eng Health Sciences, Nutrition Effect of Fatty Acid Modification on Prostacyclin Production in Cultured Human Endothelial Cells (Arachidonate, Elcosapentaenoate) text Food Science Food Science University of Illinois at Urbana-Champaign Dissertation Ph.D.