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Selvin, Paul R. Snyder, Gregory E. 2015-09-25T20:02:46Z 2015-09-25T20:02:46Z 10000-01-01 2003 2003 "The other protein I studied is myosin V which walks bipedally along actin filaments taking steps which displace the center of mass 37 nm per step. A controversy over the nature of this movement was settled earlier this year by other members of our laboratory when they showed that myosin V processes in a hand-over-hand manner, with each of the heads alternating in the leading and trailing positions. To do this, they developed a new technique which they called FIONA for Fluorescence Imaging with One Nanometer Accuracy which allowed them to localize individual molecules of myosin V to within 1.5 nut on the surface of a microscope cover slip. I have extended and applied FIONA to a mutant of myosin V with a molecule of the enhanced Green Fluorescent Protein (eGFP) fused to one head of the dieter. I demonstrated that eGFP is suitable to use with FIONA, confirm the hand-over-hand model of myosin procession, and argue that in aggregate, the data on myosin's steps from our lab implies that myosin V adopts an asymmetric, ""telemark skier,"" configuration between steps. (Abstract shortened by UMI.)." Made available in DSpace on 2015-09-25T20:02:46Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3101972.pdf: 4402337 bytes, checksum: 65ff7bc7588f5430d12855e5fa82cf3c (MD5) Previous issue date: 2003 Embargo set by: Seth Robbins for item 81779 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 91 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003. http://hdl.handle.net/2142/80497 (MiAaPQ)AAI3101972 eng Biophysics, General Lighting Up Life: Fluorescence Studies of the Shaker Potassium Ion Channel and Myosin v in Action text Physics Physics University of Illinois at Urbana-Champaign Dissertation Ph.D.