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oai:www.ideals.illinois.edu:2142/80532 2023-07-11 col_2142_5131 col_2142_8859 com_2142_5130 com_2142_8858 com_2142_234

Kwiat, Paul G. Peters, Nicholas A. 2015-09-25T20:02:57Z 2015-09-25T20:02:57Z 10000-01-01 2006 2006 The expression of the transgene gus was studied in all 40 callus lines, 12 regenerated T0 plants and the T1 and T2 progenies of 5 lines. The isogenic SC lines had an average expression level based on the activity of beta-glucuronidase of 158 +/- 9 units/mg protein (mean +/- SEM; n=3; variance within SC lines are expressed as standard error of the mean SEM) indicating a significantly higher level of expression, as compared to MC lines that had a much lower expression level 44 +/- 8 units/mg protein (mean +/- SEM; n=3) and the imprecise lines that had 22 +/- 8 units/mg protein (mean +/- SEM; n=3). Transgene expression in the callus cells of precise single copy lines varied by ∼3 fold, whereas that in multi-copy lines varied by ∼30 fold. Furthermore, precise single copy lines, on an average, contained ∼3.5 fold higher expression than multi-copy lines. Transgene expression in the plants of precise single-copy lines was highly variable, which was found to be due to the loss of the integration because of CRE-mediated reversion in the locus. (Abstract shortened by UMI.). Made available in DSpace on 2015-09-25T20:02:57Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3223690.pdf: 3889079 bytes, checksum: 6ff7bf876be965b86bae5adf98e31070 (MD5) Previous issue date: 2006 Embargo set by: Seth Robbins for item 81814 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 118 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006. http://hdl.handle.net/2142/80532 (MiAaPQ)AAI3223690 eng Physics, Optics One- and Two -Photon States for Quantum Information text Physics Physics University of Illinois at Urbana-Champaign Dissertation Ph.D.