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Kenis, Paul J.A. Talreja, Sameer 2015-09-25T20:43:37Z 2015-09-25T20:43:37Z 10000-01-01 2008 2008 In sum, we report new evaporation- and dilution-based protocols that will enable structural biologist to rapidly determine the phase diagram (e.g. solubility boundary, metastable zone width) of proteins of unknown structure using a very small sample volume. The knowledge of phase diagram of a protein/precipitant system thus obtained will be useful in obtaining high quality crystals for X-ray diffraction studies. Moreover, we use theory to compare different protein molecules on a generalized phase diagram using the solubility data obtained from our experiments. The comparison of the solubility boundary and the metastable boundary on the same footing will provide a reasonable estimate of the metastable zone width, which will aid crystallographers in screening conditions conducive for protein crystallization. We also develop a kinetic model that describes the competition between the rates of supersaturation, crystal nucleation and crystal growth occurring in the regulated-evaporation crystallization process. The knowledge of these rates coupled with the knowledge of the phase diagram of a protein/precipitant system will enable crystallographers to predict a priori the outcome of an experiment performed in an evaporation-based crystallization platform. Made available in DSpace on 2015-09-25T20:43:37Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3314911.pdf: 2114315 bytes, checksum: 663a387958936ce498fa024cba1de270 (MD5) Previous issue date: 2008 Embargo set by: Seth Robbins for item 83691 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 139 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008. http://hdl.handle.net/2142/82410 (MiAaPQ)AAI3314911 eng Engineering, Chemical Elucidation of Protein -Precipitant Phase Diagrams and Their Link to Crystal Quality text Chemical Engineering Chemical Engineering University of Illinois at Urbana-Champaign Dissertation Ph.D.