2026-09-18T20:17:03Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/82423 2023-07-11 col_2142_5131 col_2142_11615 com_2142_5130 com_2142_9130 com_2142_8903

Leckband, Deborah E. Maruthamuthu, Venkat 2015-09-25T20:43:58Z 2015-09-25T20:43:58Z 10000-01-01 2009 2009 NCAM forms a complex between its terminal domains Ig1 and Ig2. When NCAM of cell A and cell B connect to each other through complexes Ig12(A)/Ig12(B), the relative mobility of cells A and B and membrane tension exerts a force on the Ig12(A)/Ig12(B) complex. Here we investigate the response of the complex to force, using steered molecular dynamics. Starting from the structure of the complex from the Ig1-Ig2-Ig3 fragment, we first equilibrate the complex in solvent and show that its actual end-to-end length is markedly larger than in the crystal structure. We then show that the Ig12/Ig12 complex can behave as a molecular spring of spring constant ∼0.03 N/m in response to forces of tens of pico-Newton. Such tertiary structure elasticity can be expected to be pervasive considering the large number of multi-modular CAMs. Finally, we rupture the complex using higher forces to identify E16, F19, K98, and L175 as key residues stabilizing the complex. Made available in DSpace on 2015-09-25T20:43:58Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3363031.pdf: 3996524 bytes, checksum: 87d145e91f91a0fe627c480dcbd77bda (MD5) Previous issue date: 2009 Embargo set by: Seth Robbins for item 83704 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 151 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009. http://hdl.handle.net/2142/82423 (MiAaPQ)AAI3363031 eng Chemistry, Biochemistry Molecular Mechanisms of Cell Adhesion text Chemical Engineering Chemical Engineering University of Illinois at Urbana-Champaign Dissertation Ph.D.