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Leckband, D.E. Mann, Cynthia Marie 2015-09-25T20:44:02Z 2015-09-25T20:44:02Z 10000-01-01 2009 2009 This work describes the use of polyacrylamide hydrogels as controlled elastic modulus substrates for single cell traction force microscopy studies. The first section describes the use of EDC/NHS chemistry to convalently link microbeads to the hydrogel matrix for the purpose of performing long-term traction force studies (7 days). The final study uses the C2C12 cell line to demonstrate that integrin-mediated adhesion to soft substrates causes different cell behavior than N-cahderin-mediated adhesion to soft substrates. Cells plated on laminin-coated hydrogels exhibited stiffness dependent increases in cell spreading, whereas cells plated on N-cadherin-coated substrates. Similarly, cells plated on laminin-coated substrates exhibited substrate stiffness dependent increases in normalized net contractile moment, however the same cells plated on N-cadherin-coated substrates were unable to deform any but the softest hydrogels. Made available in DSpace on 2015-09-25T20:44:02Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3399020.pdf: 1891048 bytes, checksum: e6a06cfc95a11b0b115a628d6d40e84a (MD5) Previous issue date: 2009 Embargo set by: Seth Robbins for item 83713 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 85 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009. http://hdl.handle.net/2142/82432 (MiAaPQ)AAI3399020 eng Engineering, Materials Science Applications of Traction Force Microscopy in Measuring Adhesion Molecule Dependent Cell Contractility text Chemical Engineering Chemical Engineering University of Illinois at Urbana-Champaign Dissertation Ph.D.