2026-09-22T11:30:19Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/82488 2023-07-11 col_2142_5131 col_2142_13007 com_2142_5130 com_2142_13006

Chris Doe Peng, Chian-Yu 2015-09-25T20:45:31Z 2015-09-25T20:45:31Z 10000-01-01 2001 2001 By screening a collection of deficiency lines and testing candidate gene involved in cell polarity, we identified lethal giant larvae (lgl) and discs large (dlg) to play key roles in the basal targeting of Prospero and Miranda. In embryonic and larval neuroblasts of lgl and dlg mutants, all basal targeted proteins become uniformly localized but distributions of the apical proteins are unaffected. In neuroblasts, Lgl protein is uniformly distributed at the cell cortex, and its cortical attachment is dependent on Dlg's apically enriched localization. Pharmacological studies suggest Lgl mediated basal targeting occur via a myosin-based transport mechanism, which is supported by genetic studies that show loss of Myosin II suppresses Lgl phenotype in neuroblasts. Taken together, we conclude that Lgl may function to suppress Myosin II activity and allow/promote positive Myosin dependent asymmetric localization in neuroblasts. Made available in DSpace on 2015-09-25T20:45:31Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3017180.pdf: 7020606 bytes, checksum: add73ca4f278b8be6d65f549e3f91ad7 (MD5) Previous issue date: 2001 Embargo set by: Seth Robbins for item 83769 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 166 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001. http://hdl.handle.net/2142/82488 (MiAaPQ)AAI3017180 eng Biology, Neuroscience Mechanisms of Asymmetric Protein Localization in Drosophila Embryonic Neural Stem Cells text Neuroscience Neuroscience University of Illinois at Urbana-Champaign Dissertation Ph.D.