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oai:www.ideals.illinois.edu:2142/82493 2023-07-11 col_2142_5131 col_2142_13007 com_2142_5130 com_2142_13006

Ramirez, Victor D. Smith, Sean Michael 2015-09-25T20:45:32Z 2015-09-25T20:45:32Z 10000-01-01 2002 2002 In a third set of experiments, I assessed the effects of DARP-36aa on the survival of mesencephalic neurons in vitro. Treatment of mesencephalic cultures with DARP36aa resulted in a significant increase in neuron survival and morphological development. Mesencephalic cultures were also incubated with antibodies against DARP-36aa (antiDARP-36aa) in order to assess the effect of immunoneutralization of endogenous DARP on these cells. Mesencephalic cultures treated with anti-DARP-36aa contained significantly fewer neurons, and the number of branching points per neuron was markedly decreased compared to controls. DARP-36aa treatments significantly increased the number of catecholaminergic neurons in mesencephalic cultures while immunoneutralization of endogenous DARP significantly decreased catecholaminergic neuron number and morphological development. Importantly, DARP-36aa-mediated increases in neuron survival and development were completely attenuated by coincubation with genistein. In conclusion, findings from the aforementioned studies suggest that DARP-36aa is a novel neurotrophic factor that promotes survival and development of mesencephalic neurons. Made available in DSpace on 2015-09-25T20:45:32Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3070441.pdf: 6602936 bytes, checksum: 5114abd4ab26e0d8585768cbbb4ac160 (MD5) Previous issue date: 2002 Embargo set by: Seth Robbins for item 83774 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 186 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002. http://hdl.handle.net/2142/82493 (MiAaPQ)AAI3070441 eng Biology, Neuroscience Identification and Characterization of the Neurotrophic Properties of Dopamine -Releasing Protein (Darp) text Neuroscience Neuroscience University of Illinois at Urbana-Champaign Dissertation Ph.D.