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oai:www.ideals.illinois.edu:2142/82541 2023-07-11 col_2142_5131 col_2142_13007 com_2142_5130 com_2142_13006

Glaser, Michael Chan, Jonah R. 2015-09-25T20:45:46Z 2015-09-25T20:45:46Z 10000-01-01 2000 2000 In conjunction to the previous study, the platelet-derived growth factor (PDGF) A-chain was detected as two distinct splice variants in Schwann cell/neuronal co-cultures. Both PDGF A-chain isoforms were characterized and examined throughout the myelination process. The mRNA for the long form PDGF A-chain was found to be induced during myelin synthesis and was localized to the DRG neurons. Using an antibody generated against the exon 6 peptide, observations suggest that the splice variant is localized to the surface of axons and to certain Schwann cells that are elongated and ensheathing axons. Since the long form PDGF A-chain is thought to be retained on cellular surfaces by the binding of the basic carboxy-terminal to membrane associated heparan sulfate proteoglycans (HSPG), surface competition experiments were performed with heparin and the basic peptide encoded by exon 6. Heparin and the exon 6 peptide were both found to dramatically inhibit the initiation of myelin formation. While these results are still preliminary, they seem to suggest that the splice variant of the PDGF A-chain/HSPG complex functions as a novel neuronal signal that may regulate steroid biosynthesis in Schwann cells and ultimately lead to the initiation of myelin synthesis. Made available in DSpace on 2015-09-25T20:45:46Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9971042.pdf: 5838051 bytes, checksum: 99bfbc50a7c2a0ba107a085157593f9b (MD5) Previous issue date: 2000 Embargo set by: Seth Robbins for item 83822 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 129 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000. http://hdl.handle.net/2142/82541 (MiAaPQ)AAI9971042 eng Chemistry, Biochemistry The Regulation and Mechanism of Myelin Synthesis Involving Endogenous Steroid Hormones text University of Illinois at Urbana-Champaign Dissertation Ph.D. Neuroscience Neuroscience