2026-09-21T19:18:50Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/83077 2023-07-11 col_2142_5131 col_2142_14737 com_2142_5130 com_2142_690 com_2142_397

Skirvin, Robert M. Motoike, Sergio Yoshimitsu 2015-09-25T21:00:17Z 2015-09-25T21:00:17Z 10000-01-01 2001 2001 The main goal of this research was to transform Vitis. x labruscana L. H. Bailey ('Fredonia' and 'Niagara') and a seedling of a complex hybrid of 'Melody' (='Melody Seedling') with the gene Agl-iaaM (Agl = flower specific and developing fruit specific promoter; iaaM = indoleacetamide monooxygenase gene), which should induce seedlessness in these grape cultivars. To achieve this goal, three experiments were designed. In the first experiment, a method for the establishment and maintenance, as well as the regeneration and conversion of grapevine embryogenic cultures was established. In the second experiment, an efficient transformation method for 'Fredonia' and 'Melody Seedling' grapes, based on an Agrobacterium tumefaciens vector system, was developed. Finally, in the third experiment, 'Melody Seedling' was successfully transformed with the Agl5-iaaM gene. This is the first report of successful embryogenesis and genetic transformation with recovery of transgenic plants in V. x labruscana and 'Melody Seedling'. The techniques developed in this research will facilitate the genetic manipulation of the American grapes (V. x labruscana) and open a new scope to study and understand seedlessness in grapevines. Made available in DSpace on 2015-09-25T21:00:17Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3023147.pdf: 6129421 bytes, checksum: 6e75aa0a3845ea45e8cd78f1c2d84947 (MD5) Previous issue date: 2001 Embargo set by: Seth Robbins for item 84358 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 98 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001. http://hdl.handle.net/2142/83077 (MiAaPQ)AAI3023147 eng Agriculture, Plant Culture Developing a Genetic Transformation Protocol for American Grapes (Vitis X Lambruscana) and Transforming Vitis to Produce Parthenocarpic Fruits text Natural Resrouces and Environmental Sciences Natural Resrouces and Environmental Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.