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oai:www.ideals.illinois.edu:2142/83084 2023-07-11 col_2142_5131 col_2142_14737 com_2142_5130 com_2142_690 com_2142_397

Korban, Schuyler S. Li, Xiangqian 2015-09-25T21:00:20Z 2015-09-25T21:00:20Z 10000-01-01 2002 2002 The effects of different concentrations of kanamycin, carbencillin, cefotaxime, and combinations of carbencillin and cefotaxime on regeneration from different tissues of rose (Rosa hybrida cv. Carefree Beauty) were investigated. Agrobacerium tumefaciens strain GV3101 containing pCAMBIA 2301 plasmid was used for transformation. A transformation protocol by taking advantage of a secondary somatic embryogenesis system was developed. Then, a microbial protein gene, Ace-AMP1, was introduced into Rosa hybrida cv. Carefree Beauty via Agrobacterium-mediated transformation. These lines were confirmed for stable integration of Ace-AMP1 and npt II genes by PCR, Southern blotting, and Northern blotting. Transgenic rose lines inoculated with conidial spores of Sphaerotheca pannosa (Wallr.: Fr.) Lev. var. rosae showed enhanced resistance to powdery mildew. Made available in DSpace on 2015-09-25T21:00:20Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3044160.pdf: 5653987 bytes, checksum: 2ae5dad2dcf49e8e64b43322ef028181 (MD5) Previous issue date: 2002 Embargo set by: Seth Robbins for item 84365 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 143 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002. http://hdl.handle.net/2142/83084 (MiAaPQ)AAI3044160 eng Agriculture, Plant Culture Regeneration, DNA Methylation, and Transformation in Rosaceae text Natural Resrouces and Environmental Sciences Natural Resrouces and Environmental Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.