2026-09-21T13:11:25Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/83101 2023-07-11 col_2142_5131 col_2142_14737 com_2142_5130 com_2142_690 com_2142_397

Korban, Schuyler S. Schaefer, Scott Charles 2015-09-25T21:00:29Z 2015-09-25T21:00:29Z 10000-01-01 2004 2004 The influence of three different agar concentrations, 0.8, 1.0, and 1.2%, on tomato transformation efficiency was examined. Cotyledons of Lycopersicon esculentum cv. 'Sweet Chelsea' were cocultivated with Agrobacterium tumefaciens strain GV3101 harboring derivatives if the binary vector pCAMBIA 2301. The binary vectors pGR-1, pChi-1, pRIP-1, and pAFP-1 carried a maize beta-glucanase, maize chitinase, iris ribosome-inactivating protein (RIP), or an antimicrobial protein gene, respectively, each driven by the CaMV 35S promoter. Transformation was initially verified by the presence of an nptII gene and GUS enzyme activity in the uidA reporter gene, both engineered into the gene construct. Further confirmation of the transgenics was accomplished by Southern blot hybridization. Lines containing the transgenes were exposed to the tomato fungal pathogen Alternaria solani. The plants were assessed for pathogen susceptibility. Selected beta-glucanase and Mj-AMP lines were more resistant to early blight than non-transformed lines. Made available in DSpace on 2015-09-25T21:00:29Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3160949.pdf: 6722512 bytes, checksum: dfbceb6848ea11b4a9f6aa08a71ababd (MD5) Previous issue date: 2004 Embargo set by: Seth Robbins for item 84382 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 115 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004. http://hdl.handle.net/2142/83101 (MiAaPQ)AAI3160949 eng Agriculture, Plant Pathology Characterization and Expression of Plant Defense Genes in Peach and Tomato text Ph.D. Natural Resrouces and Environmental Sciences Natural Resrouces and Environmental Sciences University of Illinois at Urbana-Champaign Dissertation