2026-09-18T20:15:27Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/83113 2023-07-11 col_2142_5131 col_2142_14737 com_2142_5130 com_2142_690 com_2142_397

10000-01-01 Skirvin, Robert M. Mulwa, Richard Mwanza 2015-09-25T21:00:33Z 2015-09-25T21:00:33Z 2005 2005 There is an emerging and fast-growing viticulture industry in Illinois and the greater Midwestern U.S.A. However, one of the greatest factors limiting the establishment of vineyards in this region is the widespread use of 2,4-D, an effective herbicide used in the control of broadleaf weeds in grain crops and home lawns. 2,4-D easily drifts and severely affects grape plantings around areas where it is used. Since grapes have no known genetic resistance to this herbicide, breeding to incorporate resistance in commercial cultivars cannot be done. Thus genetic engineering has to be employed to produce adaptable grape cultivars. The overall objective of this study was to incorporate the 2,4-D resistance gene, tfdA, from Ralstonia eutrophus into grapes and produce transgenic plants that could tolerate exposure to 2,4-D. To achieve this goal a plant regeneration system based on somatic embryogenesis was developed for the 'Chancellor' wine grape. Secondly, an Agrobacterium transformation vector was constructed and its effectiveness demonstrated by the incorporation of the tfdA gene into tobacco. The vector was used to transform 'Chancellor' grape embryogenic cells and transgenic plant regenerated from these cells. Subsequently the transgenic status of the plants was proved by PCR and southern blot techniques. Spray tests with a commercial formulation of 2,4-D showed that the plants were resistant to up to 20 times the 2,4-D application rate recommended for corn. This is the first attempt at engineering 2,4-D resistance in grapes. Made available in DSpace on 2015-09-25T21:00:33Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3202146.pdf: 4336121 bytes, checksum: 5dd76345dae5a4f91317783136f29ecb (MD5) Previous issue date: 2005 Embargo set by: Seth Robbins for item 84394 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 141 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005. http://hdl.handle.net/2142/83113 (MiAaPQ)AAI3202146 eng Biology, Plant Physiology Genetic Engineering of 2,4-D Resistance in Wine Grapes text Natural Resrouces and Environmental Sciences Natural Resrouces and Environmental Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.